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| 龙脊稻作梯田土壤细菌群落结构和功能类群及影响因子分析 |
| Community structure and functional groups of soil bacteria and their influencing factors in Longji rice terraces |
| Received:August 07, 2020 |
| DOI：10.13254/j.jare.2020.0413 |
| 中文关键词: 稻作梯田，细菌群落，功能类群，pH，碳氮比 |
| 英文关键词: rice terraces, bacterial community, functional group, pH, carbon–nitrogen ratio |
| 基金项目:国家自然科学基金项目（41867008，41361054）；广西自然科学基金项目（2018GXNSFAA281247）；桂林理工大学博士启动基金项目（GUTQDJJ2004041）；广西科技计划项目（桂科AD18126018） |
| Author Name | Affiliation | E-mail | | WANG Xiao-tong | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China | | | JIN Zhen-jiang | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China | zhenjiangjinjin@163.com | | ZHOU Jun-bo | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China | | | CHENG Yue-yang | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China | | | YUAN Wu | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China | | | PAN Fu-jing | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China | | | LIU De-shen | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China
Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China | |
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| 中文摘要: |
| 为研究稻作梯田土壤细菌群落结构和功能类群特征，以龙脊稻作梯田为研究对象，在19个不同海拔高度的样地采集耕层土壤样品，采用16S rRNA高通量测序法对细菌群落结构进行分析，利用FAPROTAX功能预测软件对细菌的功能类群进行预测。结果表明，龙脊稻作梯田优势门为变形菌门和绿弯菌门，丰度分别为37.40%~63.28%和11.30%~40.78%；优势属为鞘氨醇单胞菌属和罗思河小杆菌属，丰度分别为7.13%~20.87%和2.34%~21.53%。土壤细菌优势OTUs聚类热图分析显示，与细菌丰度显著相关的环境因子为pH、碳氮比、海拔和碱解氮。共预测到51个功能类群，其中，反硝化类功能类群、硫呼吸类功能类群和烃降解类功能类群受以上几种环境因子影响显著。研究表明，龙脊稻作梯田土壤pH、碳氮比、海拔和碱解氮显著影响土壤细菌群落结构和功能类群。 |
| 英文摘要: |
| The study of soil bacterial community structure and functional groups in rice terraces can provide a theoretical basis for the sustainable management of terraces. Nineteen soil samples were collected at different altitudes in Longji rice terraces. Bacterial community structure was analyzed using 16S rRNA high-throughput sequencing, and its functional group was predicted using FAPROTAX functional prediction software. The results showed that the dominant phyla were Proteobacteria and Chloroflexi with 37.40%~63.28% and 11.30%~40.78% abundance, respectively. The dominant genera were Sphingomonas and Rhodanobacter with 7.13%~20.87% and 2.34%~21.53% abundance, respectively. Clustering heatmap of dominance operational taxonomic units(OTUs) showed that the environmental factors significantly related to bacterial abundance were pH, carbon-nitrogen ratio(C/N), alkali-hydrolyzed nitrogen(AN), and altitude. A total of 51 functional groups were predicted based on all the OTUs. Denitrification, sulfur respiration, and hydrocarbon degradation functional groups were significantly related to pH, C/N, AN, and altitude. The above results indicated that soil pH, C/N, AN, and altitude significantly affected soil bacterial community structure and functional groups in Longji rice terraces. |
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