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| 一株异养硝化-好氧反硝化菌的分离鉴定及其对养殖废水中氮的去除特性 |
| Isolation and identification of a heterotrophic nitrifying and aerobic denitrifying strain and its removal characteristics of nitrogen in breeding wastewater |
| Received:January 28, 2019 |
| DOI：10.13254/j.jare.2019.0059 |
| 中文关键词: 芽孢杆菌，异养硝化，好氧反硝化，养殖废水，脱氮 |
| 英文关键词: Bacillus subtilis, heterotrophic nitrification, aerobic denitrification, cultivation sewage, nitrogen removal |
| 基金项目:肇庆市科技计划项目（2018N003）；广东省科学院项目（2019GDASYL-0401002）；广东省自然科学基金项目（2018A030313476）；广州市珠江科技新星专项（201806010065） |
| Author Name | Affiliation | E-mail | | CHEN Meng | Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangzhou 510070, China
Guangdong Bowote Biological Technology Co., Ltd., Zhaoqing 526040, China | | | LI An-zhang | Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangzhou 510070, China
Guangzhou Wangdao Biological Technology Co., Ltd., Guangzhou 510663, China | | | ZHANG Ming-xia | Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangzhou 510070, China | | | ZHU Hong-hui | Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangzhou 510070, China | zhuhh@gdim.cn |
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| 中文摘要: |
| 为了获得养殖废水中高效脱氮的菌株，采用富集培养分离的方法，从猪粪水自然曝气池污水中筛得一株具有较好脱氮功能的异养硝化-好氧反硝化菌株ZF2-3，经形态学和生理生化鉴定、16S rRNA基因序列分析、系统发育树构建和特征性扩增片段分析，鉴定其为Bacillus subtilis。在分别以硫酸铵和硝酸钠为唯一氮源的人工废水培养基中，菌株ZF2-3对氨氮和硝态氮的去除率分别为85.7%和87.2%，且不积累中间产物。优化条件后发现，菌株ZF2-3脱除氨氮最适碳源是蔗糖，最适碳氮比为15。将菌株ZF2-3应用于养殖废水脱氮，发现无论在氨氮浓度相对较低的水产养殖废水还是氨氮浓度较高的猪粪废水中，菌株ZF2-3均有较好的处理效果，使水体氨氮、总氮浓度分别降低37.7%、67.4%和34.6%、30.4%，且无中间产物累积。研究表明，菌株ZF2-3对养殖废水脱氮具有良好的应用潜力。 |
| 英文摘要: |
| In order to obtain a highly efficient nitrogen-removing strain from sewage, an enrichment culture separation method was employed. A heterotrophic nitrifying and aerobic denitrifying bacterial strain ZF2-3 with high nitrogen removal efficiency was isolated from a sewage sample collected from a naturally aerated pond of liquid swine manure. Strain ZF2-3 was identified as Bacillus subtilis based on morphology, physiological and biochemical characteristics, 16S rRNA gene sequence identity, phylogenetic analysis, and specific amplification fragment identification. In artificial wastewater media containing ammonium sulfate or sodium nitrate as sole nitrogen source, the removal rates of ammonia nitrogen and nitrate nitrogen by strain ZF2-3 were 85.7% and 87.2%, respectively, and no intermediates were accumulated. According to the results of conditional optimization, the optimum carbon source for the removal of ammonia nitrogen was sucrose. In aquaculture wastewater with relatively low concentration of ammonia nitrogen, the optimum carbon-nitrogen ratio was 15. Subsequently, strain ZF2-3 was used to denitrify breeding swine manure sewage with high ammonia nitrogen content, and exhibited a satisfactory treatment effect, significantly reducing the concentration of ammonia nitrogen and total nitrogen in the sewage. The ammonia nitrogen and total nitrogen concentrations were reduced by 37.7%, 67.4%, 34.6%, and 30.4%, respectively. Moreover, no intermediate accumulation was detected during the treatment process. The results indicate that strain ZF2-3 has excellent application potential in denitrification of breeding wastewater. |
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