不同玉米（Zea mays L.）品种根细胞壁对Pb的吸附差异及FTIR表征

秦玉莹; 沈奕昕; 陈建军

文章摘要

不同玉米（Zea mays L.）品种根细胞壁对Pb的吸附差异及FTIR表征

Differences in adsorption of Pb on root cell wall of diverse maize(Zea mays L.)cultivars and analysis of FTIR spectra

Received:October 15, 2018

DOI：10.13254/j.jare.2018.0276

中文关键词: 玉米，根细胞壁，Pb，吸附，傅立叶变换红外光谱（FTIR）

英文关键词: maize, root cell wall, lead, adsorption, Fourier transform infrared（FTIR）

基金项目:国家自然科学基金项目（41661078）；农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放基金课题（16nybcdhj）；云南省农田无公害生产创新团队基金课题（2017HC015）

Author Name	Affiliation	E-mail
QIN Yu-ying	Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agro-environment and Safe-product, Tianjin 300191, China College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China
SHEN Yi-xin	College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China
CHEN Jian-jun	College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China	chenjianjun94@126.com

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中文摘要:

为揭示不同玉米品种根细胞壁吸收累积Pb的种内差异机制，通过吸附动力学分析和FTIR（傅立叶变换红外光谱）表征，研究“会单4号”和“路单8号”两个玉米品种根细胞壁对Pb的吸附差异及发生吸附作用的主要官能团。结果表明，“会单4号”在达到平衡时吸附量为7.05 mg·g^-1，明显高于“路单8号”平衡时吸附量6.36 mg·g^-1；“会单4号”解吸率为67.41%，明显低于“路单8号”的解吸率76.95%；“会单4号”和“路单8号”根细胞壁经酯化改性后对Pb的累积吸附量分别降低了44.59%、47.41%，经氨基甲基化改性后对Pb的累积吸附量降低了28.60%、24.86%，经果胶酶改性后对Pb的累积吸附量降低了15.79%、21.75%。同时对两个玉米品种根细胞壁上Pb吸附位点进行FTIR官能团表征分析，吸附Pb后细胞壁的结构未发生改变，而部分官能团的位置和含量发了明显的变化，其中“会单4号”和“路单8号”的羟基（-OH）或氨基（-NH₂）特征峰都出现了显著的偏移，经Pb吸附处理后“会单4号”的A₃₃₈₂/A₂₉₂₁比值升高了0.53，“路单8号”的A₃₃₈₅/A₂₉₂₁比值升高了0.41，“会单4号”的变幅明显高于“路单8号”，而对应的羧基（COO-，C-O）特征峰吸光度比值变化不明显。研究表明，“会单4号”对Pb的吸附量更高，且更加牢固，不易解吸；羟基或氨基参与了细胞壁对Pb的吸附过程；“会单4号”与“路单8号”对Pb表现出的累积能力差异，主要源于细胞壁上羟基或氨基含量变化的差异。

英文摘要:

This study was conducted to examine the differences in adsorption of Pb on the root cell wall of maize "Huidan No. 4" and "Ludan No.8" and identify the main functional groups by kinetics analysis and Fourier transform infrared(FTIR)characterization. The results indicated that the adsorption capacity of "Huidan No.4" was 7.05 mg·g^-1 when the value reached equilibrium, suggesting that the adsorption capacity was significantly higher than that "Ludan No.8" which showed a value of 6.36 mg·g^-1. The desorption rate of "Huidan No.4" was 67.41%, which was significantly lower than that of "Ludan No.8" with a desorption rate of 76.95%. The cumulative adsorption capacity of Pb on the cell wall of "Huidan No.4" and "Ludan No.8" was reduced by 44.59% and 47.41%, respectively, after esterification modification. The cumulative adsorption capacity of Pb decreased by 28.60% and 24.86% after amino methylation modification and the cumulative adsorption amount of Pb decreased by 15.79% and 21.75% after pectinase modification, respectively. Furthermore, analysis of the functional groups showed that the cell wall structure did not change after Pb adsorption, but the position and content of some functional groups changed significantly, among which "Huidan No.4" and the characteristic peaks of hydroxy(-OH)or amino(-NH₂)of "Ludan No. 8" showed significant shifts. After Pb adsorption treatment, the ratio of A₃₃₈₂/A₂₉₂₁ of "Huidan No.4" increased by 0.53 and ratio of "Ludan No.8" A₃₃₈₅/A₂₉₂₁ increased by 0.41. The variation range of "Huidan No.4" was obviously higher than that of "Ludan No.8", but the difference in the absorbance ratio of the carboxyl(COO-, CO)characteristic peak was not obvious. Our results indicate that "Huidan No.4" has a higher adsorption capacity for Pb and is more firm and more difficult to desorb. The hydroxyl or amino group participates in the adsorption process of Pb on the cell wall. The difference in the cumulative ability to Pb of "Huidan No.4" and "Ludan No.8" is mainly related to differences in the change in the content of hydroxyl or amino groups in the cell wall.

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