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| 外加镉处理下秸秆生物质炭对土壤酶活性的影响 |
| Effects of Straw Biomass Charcoal on Enzyme Activity in Cd Contaminated Soil |
| Received:October 10, 2014 |
| DOI：10.13254/j.jare.2014.0266 |
| 中文关键词: 生物质炭;Cd 污染;土壤碳循环酶;土壤氧化还原酶 |
| 英文关键词: biomass charcoal;Cd pollution;soil carbon cycle enzyme;soil REDOX enzyme |
| 基金项目:云南省应用基础研究计划项目(2013FB043);国家自然科学基金(41301349) |
| Author Name | Affiliation | E-mail | | SHANG Yi-jie | College of Resource and Environment, Yunnan Agricultural University, Kunming 650201, China | | | WANG Hai-bo | College of Resource and Environment, Yunnan Agricultural University, Kunming 650201, China | | | SHI Jing | College of Resource and Environment, Yunnan Agricultural University, Kunming 650201, China | 383110966@qq.com |
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| 中文摘要: |
| 选取秸秆生物质炭为原料,通过模拟实验,探究外加镉处理下生物质炭的输入对不同类别的土壤酶活性的影响。结果表明,外加镉处理为5 mg·kg-1时,土壤碳循环相关的酶在不同生物质炭量的施入时,对Cd 污染土壤反应较为敏感的有FDA 水解酶及蛋白酶;而土壤氧化还原酶中在土壤受Cd 污染的状况下,对不同量的生物质炭的施入具有较强敏感性的酶有脲酶和磷酸酶,其中较为显著者是磷酸酶,其在外加Cd处理下及不加Cd 时变化量为79.40%;同时,对土壤中的碳循环酶、氧化还原酶及这2类酶的总体活性各求几何平均数作为衡量其综合活性的指标。其中,土壤碳循环酶酶活综合指数介于0.071~0.235 之间,在外加Cd 处理的情况下,其值最高为生物质炭用量为2.5%时的0.174,比不加生物质炭及炭用量为5%时分别高出7.4%及19.5%;氧化还原酶综合指数介于0.093~0.202,在外加Cd 处理下,其值最高为生物质炭用量2.5%时的0.131,比不加生物质炭及炭用量为5%时分别高出18.50%及28.90%;土壤综合酶指数介于0.077~0.167,在外加Cd 处理的情况下,其值最高亦为生物质炭用量2.5%时的0.108,比不加生物质炭及炭用量为5%时分别高出16.26%及28.57%。 |
| 英文摘要: |
| Selected straw biomass charcoal as raw material for the simulation, this study mainly explore the effect of different amount of biomass charcoal applied on different categories of Cd contaminated soil enzyme activity. The results showed that when the soil Cd content was 5 mg·kg-1 and soil carbon cycle enzymes was with different amount of biomass carbon, the FDA hydrolase and protease were more sensi-tive to Cd contaminated soil; and under soil oxidoreductase in the Cd polluted soil condition with different amount of biomass carbon into soil, urease enzyme and phosphatase enzyme were more sensitivitive. Phosphatase was more significant which changed 79.40% compared soil without Cd. At the same time, calculated the geometric mean of carbon cycle enzymes, oxidoreductase enzymes and the two types of the over-all enzyme were as a measure of its synthesis enzymes activity. Among them, the comprehensive index of the soil carbon cycle enzymes activi-ty lay between 0.071~0.235, and when biomass carbon intake was 2.5%, the value was 0.174, which was higher 7.4%and 19.5%respectively than the enzyme without biomass and biomass carbon dosage was 5%; the comprehensive index of the oxidoreductase enzyme lay between 0.093~0.202, and when biomass carbon intake was 2.5%, the value was 0.131, which was higher 18.50% and 28.90% respectively than the enzyme without biomass and biomass carbon dosage was 5%; the comprehensive index of the soil composite enzyme index lay between 0.077~0.167, and when biomass carbon intake was 2.5%, the value was 0.108, which was higher 16.26% and 28.57% respectively than the enzyme without biomass and biomass carbon dosage was 5%. |
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