氮素和水分对贝加尔针茅草原土壤酶活性和微生物量碳氮的影响

王杰; 李刚; 修伟明; 宋晓龙; 赵建宁; 杨殿林

文章摘要

氮素和水分对贝加尔针茅草原土壤酶活性和微生物量碳氮的影响

Effects of Nitrogen and Water on Soil Enzyme Activity and Soil Microbial Biomass in Stipa baicalensis Steppe,Inner Mongolia of North China

Received:February 13, 2014

DOI：10.13254/j.jare.2014.0026

中文关键词: 贝加尔针茅草原;氮素添加;水分添加;土壤理化性质;酶活性;微生物量碳;微生物量氮

英文关键词: Stipa baicalensis steppe;nitrogen addition;water addition;soil physico-chemical properties;enzyme activities;microbial biomass C;microbial biomass N

基金项目:国家自然科学基金项目(31170435,31000242);“十二五”国家科技计划项目(2012BAD13B07)

Author Name	Affiliation	E-mail
WANG Jie	Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agri-culture, Tianjin 300191, China
LI Gang	Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agri-culture, Tianjin 300191, China
XIU Wei-ming	Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agri-culture, Tianjin 300191, China
SONG Xiao-long	Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agri-culture, Tianjin 300191, China
ZHAO Jian-ning	Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agri-culture, Tianjin 300191, China
YANG Dian-lin	Key Laboratory of Original Agro-environment Quality of Ministry of Agriculture, Agro-Environmental Protection Institute, Ministry of Agri-culture, Tianjin 300191, China	yangdianlin@caas.cn

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中文摘要:

在内蒙古贝加尔针茅草原,分别设对照(N0)、1.5 g·m^-2(N15)、3.0 g·m^-2(N30)、5.0 g·m^-2(N50)、10.0 g·m^-2(N100)、15.0 g·m^-2(N150)、20.0 g·m^-2(N200)和30g·m^-2(N300)(不包括大气沉降的氮量)8个氮素(NH₄NO₃)梯度和模拟夏季增加降水100 mm的水分添加交互试验,研究氮素和水分添加对草原土壤养分、酶活性及微生物量碳氮的影响。结果表明:氮素和水分添加对草原土壤理化性质和生物学特性有显著影响。随施氮量的增加土壤总有机碳、全氮、硝态氮、铵态氮含量呈增加的趋势,相反,土壤pH值呈降低的趋势。土壤脲酶和过氧化氢酶的活性随施氮量的增加而升高,多酚氧化酶则随施氮量的增加呈下降的趋势。氮素和水分添加对草原土壤微生物量碳氮含量有显著影响,高氮处理(N150、N200和N300)显著降低了微生物碳含量,微生物氮含量随施氮量的增加呈上升趋势。水分添加能够减缓氮素添加对微生物的抑制作用,提高微生物量碳、微生物量氮含量。草原土壤养分、土壤酶活性及土壤微生物量碳氮含量间关系密切,过氧化氢酶与全氮、总有机碳、硝态氮呈显著正相关,多酚氧化酶与铵态氮、硝态氮、全氮呈显著负相关。微生物量氮含量与土壤全氮、铵态氮、硝态氮含量以及过氧化氢酶和磷酸酶活性呈显著正相关,与多酚氧化酶呈负相关;微生物量碳与过氧化氢酶呈负相关,与多酚氧化酶活性呈正相关。

英文摘要:

In this paper, eight nitrogen treatments were applied at 0 g·m ^-2(N0), 1.5 g·m ^-2(N15), 3.0 g·m ^-2(N30), 5.0 g·m ^-2(N50), 10.0 g·m ^-2(N100), 15.0 g·m ^-2(N150), 20.0 g·m ^-2(N200), 30.0 g·m ^-2(N300) as NH ₄ NO ₃ and adding water to simulate summer rainfall of 100 mm, the interactive experiment was set to explore the effects of nitrogen and water addition in Stipa baicalensis steppe on soil nutrients, enzyme activities and soil microbial biomass. The results showed that the nitrogen and water addition changed soil physico-chemical factors obviously, the content of soil total organic carbon, total nitrogen, nitrate nitrogen and ammonium nitrogen increased along with the increasing of application rate of nitrogen, on the contrary, the soil pH value had decreasing trend. Appropriate application of nitrogen could enhance the activity of urease and catalase but decreased the activity of polyphenol oxidase. Nitrogen and water addition had significant effect on soil microbial biomass C and N. Higher level of N fertilizer significantly reduced microbial biomass C, and the microbial biomass N was on the rise with the application rate of nitrogen. The addition of water could slow the inhibition of nitrogen to microorganism and increase the microbial biomass C and N. A closed relationship existed in soil nutrient, activities of soil enzyme and soil microbial biomass C and N. The significantly positive correlation existed between total N, organic C, nitrate N and catalase, significantly negative correlation between nitrate N, ammonium N, total N and polyphenol oxidase. Microbial biomass N was significantly positive correlated with total N, nitrate N, ammonium N, catalase, phosphatase, and was negative correlated with polyphenol oxidase. Microbial biomass C was significantly positive correlated with polyphenol oxidase, and was negative correlated with catalase.

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