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| 外源菌剂对茄子秸秆原位堆肥微生物群落结构影响及其相关性分析 |
| Exogenous microbial agents improve the microbial community structure during eggplant straw in situ composting |
| Received:June 29, 2022 Revised:September 30, 2022 |
| DOI：10.13254/j.jare.2022.0420 |
| 中文关键词: 微生物菌剂，秸秆，原位堆肥，微生物群落，相关性分析 |
| 英文关键词: microbial agents, straw, in situ composting, microbial community, correlation analysis |
| 基金项目:国家重点研发计划项目（2021YFD1700700） |
| Author Name | Affiliation | E-mail | | LI Xuefei | Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing 100081, China
Key Laboratory of Agricultural Film Pollution Prevention and Control of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China | | | JIN Tuo | Agricultural Ecology and Resource Protection Station of the Ministry of Agriculture and Rural Affairs, Beijing 100125, China | | | SHAO Mingna | Heilongjiang Agricultural Technology Promotion Center of Hulin City, Hulin 158418, China | tgz5990023@126.com | | YIN Junhua | Shandong Qingtian Plastic Industry Co., Ltd., Linzi 255410, China | | | DING Chaowu | Shandong Chengju Agricultural Technology Co., Ltd., Weifang 262700, China | | | LIU Qin | Institute of Environment and Sustainable Development in Agriculture, CAAS, Beijing 100081, China
Key Laboratory of Agricultural Film Pollution Prevention and Control of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China | liuqin02@caas.cn |
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| 中文摘要: |
| 为明确添加外源微生物菌剂对设施蔬菜秸秆参与原位堆肥的影响，以茄子秸秆为堆肥原料，通过设置添加A微生物菌剂300 kg·hm-2（a1），不添加微生物菌剂（ct），添加B微生物菌剂300（b1）、600（b2）、900 kg·hm-2（b3）5组处理，进行30 d蔬菜大棚原位堆肥试验，测定堆肥理化指标，并通过16S rDNA高通量测序技术分析堆肥过程细菌群落结构。结果表明：添加微生物菌剂能够提高堆肥温度峰值，提高堆肥期间pH下降和电导率上升的速率，显著提高全磷含量（P<0.05），加快堆肥腐熟。堆肥后全氮含量比堆肥前提高，a1、ct、b1、b2处理的全氮含量分别上升了10.8%、11.6%、33.0%、18.5%，b3处理堆肥前后全氮含量无变化。堆肥结束时，处理a1、ct、b1、b2和b3的全磷含量分别为2.0%、2.0%、2.1%、2.2%和2.1%，b2处理显著高于其他处理（P<0.05）。添加微生物菌剂没有改变堆肥土壤优势菌群的门类，但显著改变了优质菌群的相对丰度，以及优势菌属的种类和相对丰度（P<0.05）。冗余分析结果表明全氮含量与厚壁菌门（Firmicutes）相对丰度相关性较强，全磷含量、电导率与放线菌门（Actinobacteria）相对丰度相关性较强。相关性分析结果表明电导率、含水量和全磷含量是对耕层堆肥微生物优势菌群影响最显著的三个因素。研究表明，添加微生物菌剂有利于提高微生物群落多样性，对堆肥腐熟度和土壤养分的提升具有显著的促进作用。 |
| 英文摘要: |
| In this study, eggplant straw was used as the raw composting material. Treatments with 300 kg·hm-2 of microbial inoculant A(a1); no microbial inoculant(ct); and 300(b1), 600 kg·hm-2(b2), and 900 kg·hm-2(b3) of microbial inoculant B were used to conduct 30-day in situ composting experiments in vegetable greenhouses. The physical and chemical indicators of compost were determined, and the bacterial community structure during the composting process was analyzed using 16S rDNA high-throughput sequencing technology. The results demonstrated that the addition of microbial inoculants could increase the peak temperature of the compost, increase the rate of pH drop and conductivity rise during composting, significantly increase the total phosphorus content(P<0.05), and accelerate compost maturity. The total nitrogen content of groups a1, ct, b1, and b2 increased by 10.8%, 11.6%, 33.0%, and 18.5%, respectively, and those of b3 group did not change after composting. The total phosphorus content of treatments a1, ct, b1, b2, and b3 were 2.0%, 2.0%, 2.1%, 2.2%, and 2.1% after composting, respectively, and treatment b2 was significantly higher than that of the other treatments(P<0.05). Microbial inoculants did not transform the categories of dominant flora but significantly transformed the proportion of high-quality flora and the genus and abundance of dominant bacteria in the compost soil(P<0.05). Redundancy analysis(RDA) revealed that total nitrogen was strongly correlated with Firmicutes, and total phosphorus and electrical conductivity were strongly correlated with Actinobacteria. Spearman analysis demonstrated that electrical conductivity, water content and total phosphrous content were the three most significant factors associated with the predominant microbial flora in topsoil compost. Our results indicate that the addition of microbial inoculants is beneficial to improve the proportion of high-quality flora, enhance the diversity of microbial communities. |
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