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| 耐镉邻苯二甲酸二辛酯降解菌的筛选及特性研究 |
| Isolating and characteristics of a Cd-resistant microorganism used in the biodegradation of di-n-octyl phthalate |
| Received:March 13, 2020 |
| DOI：10.13254/j.jare.2020.0114 |
| 中文关键词: 耐镉，邻苯二甲酸二辛酯，生物降解，生丝微菌属 |
| 英文关键词: cadmium resistance, di-n-octyl phthalate, biodegradation, Hyphomicrobium sp. |
| 基金项目:湖南省自然科学基金（2020JJ5321）；湖南省农业农村厅2020年农业科研计划 |
| Author Name | Affiliation | E-mail | | LIU Biao | Hunan Microbiology Institute, Changsha 410009, China | | | YU Meng-yuan | Hunan Microbiology Institute, Changsha 410009, China | | | WANG Zhen | Hunan Microbiology Institute, Changsha 410009, China | | | DU Dong-xia | Hunan Microbiology Institute, Changsha 410009, China | | | CHEN Wei | Hunan Microbiology Institute, Changsha 410009, China | | | XU Li-juan | Hunan Microbiology Institute, Changsha 410009, China | | | WU Ying-ben | Hunan Microbiology Institute, Changsha 410009, China | | | WU Min-xi | Hunan Microbiology Institute, Changsha 410009, China | | | LIU Hui-zhi | Hunan Microbiology Institute, Changsha 410009, China | | | YIN Hong-mei | Hunan Microbiology Institute, Changsha 410009, China | yyqhmm@163.com |
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| 中文摘要: |
| 为获得既耐受重金属镉又能高效降解邻苯二甲酸二辛酯（DOP）的微生物菌株，利用选择培养基从污染土壤中分离高效降解微生物，并对其菌种分类和降解特性进行分析。共筛选获得5株耐镉的DOP降解菌株，其中PD-2对DOP的降解效率最高，120 h后降解率可达93.1%。结合形态学特征和16S rDNA序列分析，鉴定该菌为生丝微菌属（Hyphomicrobium sp.）。菌株PD-2对液体培养基中DOP的降解依赖于其生物量的增加，其对DOP的作用浓度和耐镉浓度范围广，可高效降解100~800 mg·L-1范围的DOP（降解率大于80%），并在镉浓度0~600 mg·L-1的培养基中生长良好。PD-2可利用邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酸二（2-乙基己基）酯（DEHP）、邻苯二甲酸二辛酯（DOP）和邻苯二甲酸（PA）作为底物生长，底物种类范围广。添加PD-2到镉和DOP复合污染土壤中，其对DOP具有显著的降解作用，PD-2在镉和DOP复合污染土壤的修复方面具有潜在的应用价值。 |
| 英文摘要: |
| To produce highly efficient di-n-octyl phthalate(DOP)-degrading microorganisms resistant to Cd, effective strains were screened out using selective media with DOP as the sole carbon source. The phylogenetic position and degradation characteristics of PD-2 were analyzed. Five strains of DOP-degrading and Cd-resistant bacteria were isolated from phthalate-contaminated soil. The strain PD-2 degraded DOP by more than 93.1% within 120 h, which was more than other strains did in the same time period. Based on its morphology and a 16S rDNA sequence analysis, strain PD-2 was identified as Hyphomicrobium sp. The DOP degradation rate changed in relation to the strain growth. PD-2 could efficiently degrade DOP over a wide range of DOP and Cd concentrations(100~800 mg·L-1 and 0~600 mg·L-1, respectively). Furthermore, PD-2 could utilize dimethyl phthalate, diethyl phthalate, di-n-butyl phthalate, di(2-ethylhexyl) phthalate, DOP, and phthalic acid as carbon sources, suggesting that PD-2 had a broad substrate spectrum. The inoculation of PD-2 to contaminated soil could markedly increase the rate at which DOP could be removed from soil. These results indicate that PD-2 is an efficient strain of Hyphomicrobium sp. for use in biodegradation and has potential for application in the bioremediation of cadmium and phthalate compoundcontaminated soil. |
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