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| 番茄秸秆固定化芽孢杆菌M1对3环PAHs污染老化土壤修复效果 |
| Remediation effects of 3-ring PAH-contaminated soil by immobilized Bacillus sp. M1 with tomato straw in coal mining area |
| Received:September 06, 2018 |
| DOI：10.13254/j.jare.2018.0225 |
| 中文关键词: 番茄秸秆，游离菌，固定化，芽孢杆菌，3环PAHs，土壤酶活性 |
| 英文关键词: tomato straw, free bacteria, immobilized bacteria, Bacillus sp., 3-ring PAHs, soil enzyme activity |
| 基金项目:现代农业产业技术体系河北省创新团队建设项目；河北省人力资源和社会保障厅项目（C2013003022）；河北省教育厅项目（Z2013058） |
| Author Name | Affiliation | E-mail | | LI Yan | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China | | | LI Cheng | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China | | | ZHANG Xiao-xue | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China | | | FENG Xuan | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China | | | WANG Wei | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China | | | FENG Sheng-dong | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China | | | NING Guo-hui | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China | | | WANG Xiao-min | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China | | | YANG Zhixin | School of Resources and Environmental Science, Hebei Agricultural University, Baoding 071001, China
Hebei Key Laboratory of Farmland Ecological Environment, Baoding 071001, China | yangzhixin@126.com |
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| 中文摘要: |
| 为探讨固定化微生物对煤矿区3环PAHs污染老化土壤的修复效果，以番茄秸秆为固定化载体材料，通过“吸附-包埋-交联法”形成了固定化芽孢杆菌微球，并采用土培试验对煤矿区土壤3环PAHs去除进行研究。结果表明，游离芽孢杆菌M1对煤矿区污染老化土壤单体芴（Flu）、菲（Phe）和蒽（Anth）的去除随接菌量的增加先升高后降低。在接菌量为1%、10%、20%（体积质量比）的游离芽孢杆菌处理中，10%处理（B2M1）对土壤Phe的去除率最高，为21.35%。不同接菌量的固定化芽孢杆菌M1微球处理对3种PAHs的去除率显著高于微球基质处理，其中，接菌量20%的固定化芽孢杆菌处理（X3M1）对土壤Flu的去除率最高，达95.25%，比不含M1菌株的番茄秸秆微球基质处理（X3）提高了12.03个百分点。对比分析扣除微球基质后的固定化M1与添加同等菌量的游离M1去除结果看出，经固定化后的菌株M1比游离菌M1显著促进了对煤矿区污染老化土壤3环PAHs的去除，不同接菌量对单体Flu和Anth去除率为72.17%~75.52%和8.97%~28.88%，分别比游离菌增加了64.10~72.31个百分点和8.13~15.24个百分点，单体Phe 1%接菌量处理比游离菌提高了5.07个百分点。从土壤酶活性看，土壤过氧化氢酶活性在固定化M1三种剂量处理下均显著高于微球基质和游离菌M1处理，随剂量增加依次是游离菌处理的1.16、1.23倍和1.20倍，是微球基质处理的1.28、1.19倍和1.16倍，与3环PAHs的去除率规律相一致，而固定化M1处理多酚氧化酶、过氧化物酶和纤维素酶活性相对于游离菌处理有不同程度的降低。综上，固定化芽孢杆菌M1对土壤3环PAHs去除具有显著促进作用，为煤矿区PAHs污染老化土壤原位修复的应用提供了重要技术参数与支撑。 |
| 英文摘要: |
| Tomato stalks were used as carrier materials to prepare immobilized Bacillus sp. M1 microspheres by an adsorption-embeddedcross-linking method in this study. The remediation effects of 3-ring Polycyclic aromatic hydrocarbons (PAHs) -contaminated soil on these microspheres in a coal mining area were evaluated through soil incubation experiments. The results indicated that the removal of 3-ring PAHs (Flu, Phe, and Anth) by free M1 first increased and then decreased as the bacteria levels increased in PAH-contaminated soil in the coal mining area. Treatment with 10% (B2M1) showed the highest removal rate of Phe, which was 21.35%, among 1%, 10%, and 20% (volume by mass) treatment with free M1. The removal rate of the three types of PAHs by the immobilized M1 microspheres was significantly higher than that by the microsphere matrix. In the tomato stalk-immobilized M1 microspheres with 20% inoculation treatment (X3M1), the removal rates of Flu were higher for all three factors than with the tomato straw microsphere matrix (X3) without M1, with the removal rate increase of 12.03%. In contrast, for immobilized M1 following the subtraction of microsphere matrix with free M1, except for the 10% and 20% Phe inoculation treatment, immobilized M1 significantly promoted the removal of 3-ring PAHs. The removal rates of Flu and Anth after different inoculation treatments were 72.17%~75.52% and 8.97%~28.88%, respectively, over that of free M1, showing increases of 64.10%~72.31% and 8.13%~15.24%. Phe was higher of 5.07% at the 1% inoculation level as compared with free M1. The catalase activity of immobilized M1 was significantly higher than that of the microsphere matrix and free M1 at different doses as bacteria levels increased. Values were 1.16-, 1.23-, and 1.20-fold higher than those of free M1 and 1.28-, 1.19-and 1.16-fold higher than those of the microsphere matrix. The change in catalase activity was consistent with the removal rate of 3-ring PAHs. Compared with free bacteria, the polyphenol oxidase, peroxidase, and cellulase enzyme activities of immobilized M1 were reduced to varying degrees. In summary, immobilized Bacillus sp. M1 significantly affected the removal of soil 3-ring PAHs. The results of the present study provide important technical parameters for and support the application of in situ remediation of PAH-contaminated aging soil in coal mining areas. |
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