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| 富硒蚯蚓的培养及其硒富集作用研究 |
| The Cultivation and SeleniumEnrichment of SeleniumEnriched Earthworm |
| Received:September 25, 2014 |
| DOI：10.13254/j.jare.2014.0247 |
| 中文关键词: 赤子爱胜蚓;存活率;蚯蚓硒含量;培养条件 |
| 英文关键词: Eisenia fetida;survival rate;selenium content in earthworm;condition of culture |
| 基金项目:国家自然科学基金(31070454);公益性行业(农业)科研专项(201303106) |
| Author Name | Affiliation | E-mail | | SUN Xiao-fei | 中国农业大学资源与环境学院, 北京100193 | | | QIAO Yu-hui | 中国农业大学资源与环境学院, 北京100193 | qiaoyh@cau.edu.cn | | SUN Zhen-jun | 中国农业大学资源与环境学院, 北京100193 | | | WANG Chong | 中国农业大学资源与环境学院, 北京100193 | | | LI Hua-fen | 中国农业大学资源与环境学院, 北京100193 | | | YUE Shi-zhong | 中国农业大学资源与环境学院, 北京100193 | |
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| 中文摘要: |
| 以赤子爱胜蚓为生物载体,在蚯蚓饲料中添加亚硒酸钠进行富硒蚯蚓培养试验,利用蚯蚓的生物富集作用将无机硒转化为有机硒。以存活率、硒含量为优化指标,研究5种硒浓度(20、40、60、80、100 mg·kg-1)和4种培养时间(第15、30、45、60 d)对蚯蚓生长和硒含量的影响规律,筛选存活率高、硒含量高、培养时间短的富硒蚯蚓培养方法。试验结果表明:蚯蚓饲料中亚硒酸钠含量越高、培养时间越长,蚯蚓的存活率越低,硒含量越高,且当硒添加量为80 mg·kg-1,培养时间为45 d时,培养条件最优,此时蚯蚓体内硒含量可达33.25 mg·kg-1。 |
| 英文摘要: |
| As a bio-carrier, Eisenia fetida was fed with cow dung that added with sodium selenite in order to transfer inorganic selenium(Se) into organic selenium. Targeting on survival rate and selenium content, the effects of five Se concentrations(0, 20, 40, 60, 80, 100 mg·kg-1) and four cultivation periods(15, 30, 45, 60 d)on earthworm growth and Se contents were investigated. The cultivation method with high survival rate, high Se content of earthworm and short breeding time would be screened out. The experimental results showed that the earthworm survival rate decreased and the Se content in earthworm increased with the increase of Se application and the extension of breeding time. The most optimummethod was screened out when the Se concentration was 80 mg·kg-1 and the cultivation period was 45 days, Se content in earthworm was up to 33.25 mg·kg-1. |
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