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| 不同碳氮源对Paracoccus sp.QD15-1降解邻苯二甲酸二甲酯的影响 |
| Effect of different carbon and nitrogen sources on degradation of phthalate degrading bacteria |
| 投稿时间：2018-08-27 |
| DOI：10.13254/j.jare.2018.0217 |
| 中文关键词: Paracoccus sp.QD15-1，邻苯二甲酸二甲酯，降解，碳源，氮源 |
| 英文关键词: Paracoccus sp.QD15-1, dimethyl phthalate, degradation, carbon source, nitrogen source |
| 基金项目:国家自然科学基金项目（31670375） |
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| 中文摘要: |
| 邻苯二甲酸二甲酯（Dimethyl phthalate，DMP）是一种高毒性的有机污染物，Paracoccus sp.QD15-1能够高效降解DMP，碳氮源是微生物需求量最大的生长要素。本实验以Paracoccus sp.QD15-1为研究对象，在基础无机盐（MSM）培养基中分别外加不同碳氮源（碳源分别为5 g·L-1的葡萄糖、蔗糖、乳糖和淀粉；氮源分别为5 g·L-1的硫酸铵、硝酸钠、硝酸铵和尿素），研究Paracoccus sp.QD15-1生长和降解DMP能力的变化。结果表明，外加4种碳源后，菌株生物量和降解DMP能力均显著提高，其中外加淀粉处理的最大比生长率达到0.13 lg（cfu）·h-1，36 h降解率为78.2%，DMP半衰期为15.6 h。外加4种氮源后，降解菌的生物量均有所上升，其中外加尿素，36 h降解率为62.8%。研究表明，Paracoccus sp.QD15-1在不同碳氮源中均能够较好地降解DMP，且外加淀粉作为碳源和外加尿素作为氮源的降解效果更佳，在实际工程应用中具有良好前景。 |
| 英文摘要: |
| Dimethyl phthalate (DMP) is a highly toxic organic pollutant. Paracoccus sp.QD15-1 can degrade DMP efficiently. The carbon and nitrogen sources are the important nutrients for the growth of microorganisms. In this study, the growth and DMP degradation of Paracoccus sp. QD15-1 were detected in the basic inorganic salt medium by addition of different carbon and nitrogen sources. The results showed that the growth and capacity for degrading DMP of Paracoccus sp.QD15-1 were significantly improved by four carbon sources. With starch addition, the maximum specific growth rate reached to 0.13 lg (cfu)·d-1, the degrading rate of DMP reached 78.2%, and half-life of DMP reached at 15.6 h. The biomass of Paracoccus sp.QD15-1 was improved significantly by the addition of nitrogen sources. With urea addition, the degrading rate of DMP reached 62.8% at 36 h. Therefore, the addition of carbon and nitrogen sources could improve the capacity of Paracoccus sp.QD15-1 for degrading DMP, and Paracoccus sp.QD15-1 would have good prospects in practical engineering applications. |
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