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| pqq基因簇在Escherichia coli DH5α中表达及对其溶磷促生的影响 |
| Expression pqq Gene Cluster and Its Effects on Mineral Phosphate Solubilization and Plant Promotion in Escherichia coli DH5α |
| 投稿时间：2015-07-16 |
| DOI：10.13254/j.jare.2015.0176 |
| 中文关键词: pqq基因簇 DH5α(pqq)菌株 矿质磷 有机酸 溶磷促生 |
| 英文关键词: pqq gene cluster strain DH5α(pqq) mineral phosphate organic acid phosphate solubilization and promotion |
| 基金项目:新疆自治区高校科研计划项目(XJEDU2014I041) |
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| 中文摘要: |
| 为丰富植物促生菌的构建模式,本文以Escherichia coli DH5α为供试菌株,通过遗传转化,将水生拉恩氏菌(Rahnella aqautilis)HX2的pqq基因簇导入E. coli DH5α,获得DH5α(pqq)菌株,采用平板溶磷、钼锑抗比色法、HPLC法、温室盆栽等试验分析研究DH5α(pqq)菌株溶磷产酸促生作用机理。DH5α(pqq)菌株与E. coli DH5α相比,溶磷圈直径、有效磷浓度增加,溶解矿质磷的能力显著增强;有机酸代谢显著增加,以产葡萄糖酸为主。DH5α(pqq)处理与对照相比对玉米株高、茎鲜重、植株鲜重、茎干重、植株干重、全磷、土壤有效磷分别提高了5.2%、30.0%、32.5%、18.5%、7.7%、30.8%和24.0%,并呈显著性差异(P<0.05)。表明来自HX2菌株的pqq基因簇通过异源表达具有活性,能够实现对溶磷促生的遗传改造。 |
| 英文摘要: |
| In this study, in order to increase the ability of phosphate solubilization of Escherichia coli DH5α, pqq gene cluster from Rahnella aqautilis HX2 was transformed into E. coli DH5α to create DH5α(pqq). Mechanisms of E. coli DH5α and DH5α(pqq) phosphate solubilization were analyzed by using solubilzing phospate on plate, molybdenum-blue method, HPLC, and greenhouse experiment. Compared with E. coli DH5α, strain DH5α(pqq) showed stronger mineral phosphate solubilizing ability, more gluconic acid production. Maize plant height, shoot fresh weight, plant fresh weight, shoot dry weight, plant dry weight, total phosphorus and soil solubling-P of DH5α(pqq) treatment were significantly(P<0.05) improved by 5.2%, 30.0%, 32.5%, 18.5%, 7.7%, 30.8%, and 24.0% respectively, compared with DH5α treatment. It was shown that pqq gene cluster from R. aqautilis HX2 strain showed activity in E. coli DH5α. This method may modify the other plant growth promoting rhizobacteria on phosphate solubilization trait. |
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