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| AA3型连续流动分析仪与钒钼黄比色法测定玉米植株全磷含量之比较 |
| Determination Total Phosphour of Maize Plant Samples by Continuous Flow Analyzer in Comparison with Vanadium Molybdate Yellow Colorimetric Method |
| 投稿时间：2015-01-29 |
| DOI：10.13254/j.jare.2015.0030 |
| 中文关键词: 连续流动分析仪 钒钼黄比色法 植物全磷 |
| 英文关键词: continuous flow analyzer (CFA) vanadium molybdate yellow colorimetric method (VMYC method) plant phosphorus |
| 基金项目:国家重点基础研究发展计划(“973”计划)项目(2013CB127402);中央级公益性科研院所专项资金资助项目(IARRP-2014-39) |
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| 中文摘要: |
| 选择69个玉米秸秆和籽粒样品,经浓H2SO4和H2O2消煮后,用连续流动分析仪与钒钼黄比色法分别测定消煮液中磷含量,对2种方法检测数据之间进行比较分析,探讨利用连续流动分析仪测定植株样品消煮液中磷含量的可行性。t检验表明:2种方法测定植株全磷含量相比无明显差异。测定结果之间呈显著线性相关系,回归直线方程为Y(连续流动分析仪-P)=0.927X(钒钼黄比色法-P)-0.002,相关系数r=0.985(n=69,P<0.01)。连续流动分析仪测定加标回收率在99.3%~103.8%之间,对4个样品消煮液中磷浓度分别重复测定10次,相对标准偏差小于1.5%。连续流动分析仪分析速度快,消耗试剂少,推荐用于大批量玉米植株样品H2SO4-H2O2消煮液中磷含量分析。 |
| 英文摘要: |
| The vanadium molybdate yellow colorimetric method(VMYC method) is regarded as one of conventional methods for determining total phosphorus(P) in plants, but it is time consuming procedure. Continuous flow analyzer(CFA) is a fluid stream segmentation technique with air segments. It is used to measure P concentration based on the molybdate-antimony-ascorbic acid method of Murphy and Riley. Sixty nine of maize plant samples were selected and digested with H2SO4-H2O2. P concentrations in the digests were determined by CFA and VMYC method, respectively. The t test found that there was no any significant difference of the plant P contents measured by the CFA and the VMYC method. A linear equation could best describe their relationship: Y(CFA-P)=0.927X(VMYC-P)-0.002. The Pearson's correlation coefficient was 0.985 with a significance level(n=69, P<0.01). The CFA method for plant P measurement had a high precision with relative standard deviation(RSD) less than 1.5%. It is suggested that the CFA based on Murphy and Riley colorimetric detection can be used to determinate total plant P in the digests solutions with H2SO4-H2O2. The CFA method is labor saving and can handle large numbers of samples. The human error in mixing with other operations is reduced to a great extent. |
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