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| 发酵床垫料中高效纤维素降解菌的分离与筛选 |
| Isolation and Identification of Cellulose-Decomposing Microorganisms in Deep-litter System |
| 投稿时间：2015-01-06 |
| DOI：10.13254/j.jare.2015.0007 |
| 中文关键词: 发酵床垫料 纤维素降解菌 酶活 鉴定 |
| 英文关键词: deep-litter system cellulose-decomposing microorganism enzyme activity identification |
| 基金项目:湖南省科技厅重大专项(2009FJ1005);湖南省生猪产业技术体系生猪产业规模养殖与环境控制岗位项目 |
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| 中文摘要: |
| 为获得高效纤维素降解菌,从发酵床垫料中分离出5 株能降解纤维素的微生物菌株,对5株菌进行了透明圈和纤维素酶活性测定。结果表明:5 株菌株均可在纤维素-刚果红平板上快速形成透明圈,其中菌株A3 产酶活性最强,在接种后72 h 达到125.47U·mL-1。对其16SrDNA 序列进行分析,结合形态学观察和生理生化特征,将该菌鉴定为地衣芽孢杆菌 (Bacillus licheniformis) 。培养时间和温度对菌株的酶活力影响较大,各菌株的产酶最适温度为30~40℃。该芽孢杆菌分解纤维素能力强、适应能力高,在发酵床垫料堆肥中具有潜在的应用前景。 |
| 英文摘要: |
| To obtain effective cellulose-degrading microorganisms, five cellulose-decomposing strains were isolated from deep-litter system and their enzyme activities were determined by DNS method. The results showed that all of the strains could form the clear zone rapidly on the cellulose-congo red medium. The strain A3 showed the highest enzyme activity and reached 125.47 U·mL-1 in 72 h after inoculation. Based on the physiological, biochemical characterization and 16S rDNA analysis, the strain A3 was identified as Bacillus licheniformis. The enzyme activity was influenced by culture temperature and time. The optimal temperature for cellulase activity was 30~40℃. Overall, with the high capacity to decompose the cellulose and adaptability to environment, the Bacillus strain would have a great potential to be applied in utilizing the cellulose. |
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