文章摘要
乙草胺降解菌WN-3的分离鉴定及其降解特性分析
Isolation, Identification and Degradation Characteristics of Acetochlor Degradation Bacterium WN-3
投稿时间:2014-11-03  
DOI:10.13254/j.jare.2014.0306
中文关键词: 乙草胺  鼻疽菌属  生理生化特征  降解特性  生物测定
英文关键词: acetochlor  Burkholderia sp.  physiological and biochemical characteristics  degrading characteristics  bioassay
基金项目:黑龙江省教育厅科技项目“抗除草剂微生物的分离与鉴定”(12541253)
作者单位E-mail
田爽 哈尔滨师范大学生命科学与技术学院, 黑龙江 哈尔滨 150025  
吴秋彩 哈尔滨师范大学生命科学与技术学院, 黑龙江 哈尔滨 150025  
王晓萍 哈尔滨师范大学生命科学与技术学院, 黑龙江 哈尔滨 150025 dr_wxp@sina.com 
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中文摘要:
      从长期受农药乙草胺污染的土壤中采用富集培养技术分离得到1株能够降解乙草胺的细菌,将其命名为WN-3.通过观察该菌株的形态学特征,研究其生理生化特性以及分析其16S rDNA序列,初步将菌株WN-3鉴定为鼻疽菌属(Burkholderia sp.).并通过研究培养时间、温度、初始pH值、接种量和乙草胺浓度对菌株WN-3的生长和降解效果的影响,确定了最佳生长和降解条件.结果显示,菌株WN-3在温度35 ℃、pH值6.0、接种量为10%、乙草胺浓度为50 mg·L-1的条件下,培养7 d后对乙草胺的降解率可达到38.3%.这为利用鼻疽菌属菌株降解农药乙草胺,进行原位生物修复提供理论依据.
英文摘要:
      A bacterium named WN-3 which was able to degrade acetochlor, was isolated from the soil of long term applied with acetochlor by enrichment culture. Based on physiological and biochemical characteristics and 16S rDNA sequence analysis, the strain WN-3 was identified preliminarily as Burkholderia sp The effect of culture time, temperature, initial pH, inoculation amount and concentration of acetochlor on growth and degradation efficiencies were studied. The results showed that the degrading efficiency could reach 38.8% under the optimal conditions of initial pH 6.0, inoculation amount of 10%, concentration of acetochlor 50 mg·L-1, respectively, after 7 days at 35 ℃. It provided theoretical basis for using a bacteria of Burkholderia sp. in situbioremediation of soil pollution of acetochlor.
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