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| UV-B辐射胁迫下水稻旗叶氮代谢的代际响应特征 |
| Transgenerational response characteristics of nitrogen metabolism in flag leaves of rice under UV-B radiation stress |
| 投稿时间：2024-07-19 |
| DOI：10.13254/j.jare.2024.0541 |
| 中文关键词: UV-B辐射，氮代谢，水稻旗叶，代际可塑性，元阳梯田 |
| 英文关键词: UV-B radiation, nitrogen metabolism, flag leaf of rice, transgenerational plasticity, Yuanyang Terrace |
| 基金项目:国家自然科学基金项目（K1420220027） |
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| 摘要点击次数: 213 |
| 全文下载次数: 251 |
| 中文摘要: |
| 为探究元阳梯田水稻（Oryza sativa L.）旗叶氮代谢对UV-B辐射胁迫的代际响应特征，通过原位种植7.5 kJ·m-2 UV-B辐射下分别处理0、1、2、3年的水稻种子，在自然光照和7.5 kJ·m-2 UV-B辐射条件下进行田间试验，研究不同世代同强度UV-B辐射对元阳梯田水稻（白脚老粳）旗叶的氮代谢相关酶和含氮化合物的影响。结果表明：相同强度UV-B辐射胁迫下，水稻的辐射记忆和生存环境匹配时，F2世代水稻旗叶的硝态氮（NO3--N）含量较F1世代显著升高9.5%，同世代的谷氨酰胺合成酶（GS）和谷氨酸脱氢酶（GDH）较F1世代分别升高157.2%和24.0%，促进了水稻叶片氨同化进程，使得铵态氮（NH4+-N）含量较F1世代显著降低；F3世代水稻旗叶的NO3--N含量较F1世代显著升高75.3%，同世代的总游离氨基酸含量较F1世代升高38.4%，F3世代水稻的氮代谢开始产生代际适应性变化，对UV-B辐射胁迫产生了抗逆性。相同强度UV-B辐射胁迫下，不同世代水稻的辐射记忆和生存环境不匹配时，旗叶的GDH活性随环境波动变化，总游离氨基酸含量显著降低，氮代谢仍受UV-B辐射抑制，水稻植株对UV-B辐射胁迫没有产生耐受性。研究表明，UV-B辐射胁迫下，元阳梯田水稻旗叶氮代谢具有代际可塑性，通过调节不同世代水稻氮代谢中的关键酶（GS和GDH）和含氮化合物含量来促进氮代谢产生适应性变化。 |
| 英文摘要: |
| In order to explore the transgenerational response characteristics of nitrogen metabolism in flag leaves of rice（Oryza sativa L.）in Yuanyang Terraced fields to UV-B radiation stress, a field experiment was conducted to simulate 7.5 kJ·m-2 UV-B radiation on the land surface. Rice seeds irradiated by 7.5 kJ·m-2 UV-B for 0, 1, 2 and 3 years were planted in situ under natural light and 7.5 kJ·m-2 UV-B radiation. A one-year field experiment was conducted to study the effects of different generations of UV-B radiation with the same intensity on nitrogen metabolism-related enzymes and nitrogen-containing compounds in flag leaves of Yuanyang Terraced rice（Baijiao Laojing）. The results showed that under the same intensity of UV-B radiation stress, when the radiation memory and living environment of rice were matched, the nitrate nitrogen（NO3--N）content of flag leaf in F2 generation was significantly increased by 9.5% compared with that in F1 generation, and the glutamine synthetase（GS）and glutamate dehydrogenase（GDH）in the same generation were 157.2% and 24.0% higher than those in F1 generation, respectively, which promoted the process of ammonia assimilation in rice leaves. The content of ammonium nitrogen（NH4+-N）was significantly lower than that of F1 generation. The NO3--N content of flag leaf in F3 generation was significantly higher than that in F1 generation by 75.3%, and the total free amino acid content in the same generation was higher than that in F1 generation by 38.4%. The nitrogen metabolism of F3 generation rice began to produce transgenerational adaptive changes, which had resistance to UV-B radiation stress. Under the same intensity of UV-B radiation stress, when the radiation memory and living environment of different generations of rice did not match, the GDH activity of flag leaf fluctuated with the environment, the total free amino acid content decreased significantly, and the nitrogen metabolism was still inhibited by UV-B radiation. Rice plants did not develop tolerance to UV-B radiation stress. In summary, under UV-B radiation stress, the flag leaf nitrogen metabolism of Yuanyang Terraced rice has transgenerational plasticity, which promotes adaptive changes in nitrogen metabolism by regulating the key enzymes（GS and GDH）and nitrogen-containing compounds in nitrogen metabolism of different generations of rice. |
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