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| 还原型谷胱甘肽对间作体系下小花南芥铅富集特征的影响 |
| Effects of reduced glutathione on lead accumulation characteristics of Arabis alpina L. var. parviflora Franch under intercropping system |
| 投稿时间：2024-07-02 |
| DOI：10.13254/j.jare.2024.0482 |
| 中文关键词: 间作 小花南芥 谷胱甘肽 铅 抗氧化酶 |
| 英文关键词: intercropping Arabis alpina L. var. parviflora Franch GSH lead antioxidant enzyme |
| 基金项目:国家自然科学基金项目（41967049，41761073） |
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| 中文摘要: |
| 为探究还原型谷胱甘肽（GSH）对间作体系下小花南芥（Arabis alpina L. var. parviflora Franch）铅富集特征的影响，本研究设置（1 000 mg·kg-1）铅胁迫下叶面喷施不同浓度（0、5、10、15 mmol·L-1）的 GSH和 1 mmol·L-1的谷胱甘肽合成酶抑制剂（丁硫氨酸-亚砜亚胺，L-Buthionine-sulfoximine，BSO）对小花南芥生物量、光合特性、抗氧化酶活性和铅富集特征的的影响。结果表明：间作小花南芥的叶长、生物量、光合色素含量以及光合参数随着GSH浓度的增加而增加。与0 mmol·L-1浓度处理相比，10 mmol·L-1浓度处理下，间作小花南芥地上部铅含量、转运和富集系数显著增高31.04%、31.82%和31.04%，地上部细胞膜透性（REC）和丙二醛（MDA）含量显著下降58.40%和29.55%，地上部GSH活性显著增加149.66%；15 mmol·L-1浓度处理下，间作小花南芥地上部γ-谷氨酰半胱氨酸合成酶（γ-GCS）活性、超氧阴离子（O-2·）和过氧化氢（H2O2）的产生量显著下降24.02%、38.40%和36.18%，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）和氧化型谷胱甘肽（GSSG）含量显著增加 116.46%、478.81%、103.40% 和162.47%。而添加BSO则与上述结果相反。综上所述，10、15 mmol·L-1 处理下，GSH通过提高抗氧化酶活性，来缓解铅胁迫对小花南芥造成的生理损害，增加光合色素含量增强小花南芥光合作用，进而提高小花南芥抵抗铅胁迫的能力，促进其对铅的富集。 |
| 英文摘要: |
| In order to explore the effect of reduced glutathione（GSH）on lead（Pb）enrichment characteristics of Arabis alpina L. var. parviflora Franch under intercropping system, effects of foliar spraying different concentrations（0, 5, 10, 15 mmol · L-1）of GSH and 1 mmol·L-1 of glutathione synthetase inhibitor（L-Buthionine-sulfoximine, BSO）on the biomass, photosynthetic characteristics, antioxidant enzyme activities and Pb accumulation characteristics of A. alpina under Pb stress（1 000 mg·kg-1）were studied. The results showed that the leaf length, biomass, photosynthetic pigment content and photosynthetic parameters increased with the increase of GSH concentration. Compared with the 0 mmol · L-1 GSH treatment, the Pb content, translocation and enrichment coefficient of the aboveground part of the intercropping were significantly increased by 31.04%, 31.82% and 31.04% under the 10 mmol·L-1 GSH treatment. The REC and MDA in the aboveground part decreased significantly by 58.40% and 29.55%, and the GSH activities in the aboveground part increased significantly by 149.66%. Under the treatment of 15 mmol·L-1 GSH, the γ-GCS activity, O -2·and H2O2 production of intercropping were significantly decreased by 24.02%, 38.40% and 36.18%, and the contents of SOD, CAT, POD and GSSG were significantly increased by 116.46%, 478.81%, 103.40% and 162.47%. The addition of BSO was contrary to the above results. In summary, under the treatment of 10 and 15 mmol · L-1, GSH alleviates the physiological damage caused by lead stress by increasing the activity of antioxidant enzymes, increases the content of photosynthetic pigments and enhances the photosynthesis of A.alpina, thereby improving the ability of A.alpina to resist lead stress and promote its enrichment of Pb. |
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