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| 西双版纳野生稻根际细菌群落结构及功能特征分析 |
| Analysis of the structure and functional characteristics of the rhizosphere bacterial community in wild rice from Xishuangbanna，China |
| 投稿时间：2024-06-19 |
| DOI：10.13254/j.jare.2024.0436 |
| 中文关键词: 野生稻 根际细菌 土壤理化性质 群落结构 功能预测 |
| 英文关键词: wild rice rhizosphere microbiota soil physical and chemical characteristics community structure functional prediction |
| 基金项目:国家自然科学基金项目（42267062，32260314） |
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| 中文摘要: |
| 为揭示云南西双版纳野生稻原生境下根际土壤细菌的群落结构及物种多样性，本研究以云南省西双版纳采集的三种野生稻（疣粒野生稻、药用野生稻和普通野生稻）为研究对象，借助高通量测序技术，结合土壤理化性质探讨影响不同野生稻根际细菌群落组成差异的因素，并对细菌群落进行功能预测分析。结果显示，疣粒野生稻土壤有机质（SOM）、全氮（TN）、速效钾（AK）和碱解氮（AN）含量显著高于药用野生稻（P<0.05），土壤养分状况最佳。野生稻根际细菌隶属于6门3属，变形菌门（Proteobacteria）在三种野生稻中为优势菌门，相对丰度均大于70%；属水平上疣粒野生稻最大丰度的菌属为雷尔氏菌属（Ralstonia，34.53%），药用野生稻和普通野生稻为鞘氨醇单胞菌属（Sphingomonas，25.86%和29.74%）。AK、SOM含量和pH与鞘氨醇单胞菌属丰度呈正相关，全磷（TP）和AK含量与雷尔氏菌属和新鞘脂菌属丰度呈正相关（P<0.05）。碳水化合物代谢和氨基酸代谢通路是主要的功能组成，尤其是在酮体的合成与降解、细菌趋化方面较为突出。总的来说，三种野生稻根际菌群丰富度、群落结构组成及功能多样性上差异显著。研究结果丰富了云南野生稻根际细菌群落结构与功能的生物学信息，可为该物种资源保护与合理利用提供理论依据。 |
| 英文摘要: |
| To reveal the community structure and species diversity of rhizosphere soil bacteria in the original habitat of wild rice in Xishuangbanna, three wild rices（Oryza granulata Baill., Oryza officinalis Wall., Oryza rufupogon Griff.,）collected in Xishuangbanna, Yunnan Province were selected as the research subjects. Based on high-throughput sequencing technology and soil physicochemical properties, we explored the factors which affected the differences in the composition of the rhizosphere bacteria communities of three wild rice species and analyzed the functional prediction of the bacterial communities. The results showed that soil organic matter（SOM）, total nitrogen（TN）, available potassium（AK）and available nitrogen（AN）content of O. granulata were significantly higher than those of O. officinalis （P<0.05）, indicating the best nutrient status. The rhizosphere microbiota of wild rice belonged to 6 phyla and 3 genera, in which Proteobacteria was the dominant phylum in the three wild rice species with relative abundance more than 70%. The genus with the greatest abundance in O. granulata was Ralstonia（34.53%）, and in O. officinalis（25.86%）and O. rufupogon was Sphingomonas（29.74%）. AK and SOM content and pH were positively correlated with the relative abundance of Sphingomonas spp., while available（AP）and AK content were positively correlated with relative abundances of Ralstonia spp. and Sphingomonas spp.（P<0.05）. Carbohydrate and amino acid metabolism pathways were the main functional components, especially prominent in the synthesis and degradation of ketone bodies and bacterial chemotaxis. In general, significant differences exist in the richness, community structure, and functional diversity of the rhizosphere microbiota among the three wild rice species. The results enrich the biological information on the structure and function of wild rices rhizosphere bacterial communities, and may provide a theoretical basis for the conservation and rational utilization of the resources of wild rices. |
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