|
| 叶面喷施植物生长调节剂对盐碱胁迫下小麦幼苗生长及生理指标的影响 |
| Effects of foliar spraying plant growth regulator on growth and physiological indexes of wheat seedlings under saline-alkali stress |
| 投稿时间：2024-07-22 |
| DOI：10.13254/j.jare.2024.0567 |
| 中文关键词: 小麦，抗氧化酶，渗透调节，内源激素 |
| 英文关键词: wheat, antioxidant enzymes, osmoregulation, endogenous hormones |
| 基金项目:国家重点研发计划项目（2021YFD1900802）;天山英才科技创新领军人才项目（2022TSYCLJ0051）;新疆维吾尔自治区重点研发项目（2022B02020） |
| 作者 | 单位 | E-mail | | 蔡鑫 | 石河子大学, 新疆兵团绿洲生态农业重点实验室, 新疆 石河子 832003 | | | 陈虹 | 石河子大学, 新疆兵团绿洲生态农业重点实验室, 新疆 石河子 832003 | | | 汪灏然 | 石河子大学, 新疆兵团绿洲生态农业重点实验室, 新疆 石河子 832003 | | | 刘航希 | 石河子大学, 新疆兵团绿洲生态农业重点实验室, 新疆 石河子 832003 | | | 张凤华 | 石河子大学, 新疆兵团绿洲生态农业重点实验室, 新疆 石河子 832003 | zfh2000@126.com |
|
| 摘要点击次数: 430 |
| 全文下载次数: 165 |
| 中文摘要: |
| 为探究叶面喷施植物生长调节剂对盐碱胁迫下小麦苗期生长的影响，本研究采用双因素完全随机试验，设置4个不同盐碱（NaCl∶NaHCO3=1∶1）浓度处理组（0、50、100、150 mmol·L-1）以及4种叶面喷施组[清水、0.1 mmol·L-1水杨酸、0.1 mmol·L-1亚精胺、100 mg·L-1 5-氨基乙酰丙酸（5-ALA）]，分析苗期小麦抗氧化酶活性、渗透调节物质以及内源激素含量的变化。结果表明，150mmol·L-1盐碱胁迫下，与清水处理相比，其他3种叶面喷施处理小麦幼苗株高分别提高7.69%~13.15%、12.66%~15.50%和1.01%~12.68%；根长分别提高7.44%~25.28%、2.21%~19.62%和30.38%~60.52%；茎鲜质量分别提高了3.58%~16.59%、12.50%~47.61%和20.00%~96.19%。与清水处理相比，喷施生长调节剂提高了小麦幼苗抗氧化酶活性，其中喷施水杨酸对小麦幼苗抗氧化酶活性提升效果最佳。各盐碱胁迫下，与清水处理相比，喷施水杨酸、亚精胺显著增加了小麦叶片可溶性糖与可溶性蛋白含量，分别提高了13.19%~23.11%、8.99%~27.65%和3.18%~28.76%、7.50%~29.31%。0、100、150 mmol·L-1盐碱胁迫下，喷施水杨酸小麦叶片生长素含量显著高于其他处理；在50、100 mmol·L-1盐碱胁迫下，喷施水杨酸显著提高赤霉素含量；在50 mmol·L-1和150 mmol·L-1盐碱胁迫下，喷施水杨酸与亚精胺处理细胞分裂素含量显著高于5-ALA；在0 mmol·L-1和100 mmol·L-1盐碱胁迫下，喷施5-ALA显著降低叶片脱落酸含量，增加细胞分裂素含量。研究表明，盐碱胁迫下喷施水杨酸和亚精胺对小麦苗期生长的促进效果优于5-ALA，有助于缓解盐碱胁迫对其生长的抑制作用，提高小麦的耐盐碱性。 |
| 英文摘要: |
| In order to investigate the effect of foliar spraying of plant growth regulators on seedling growth of wheat under saline-alkali stress, a two-factor completely randomised trial was used in this study. Four treatment groups with different saline-alkali（NaCl∶NaHCO3= 1∶1）concentrations（0, 50, 100 mmol·L-1 and 150 mmol·L-1）and four foliar spraying groups[water, 0.1 mmol·L-1 salicylic acid, 0.1 mmol· L-1 suberic acid, 100 mmol · L-1 5-aminolevulinic acid（5-ALA）] were used to analyse the changes in antioxidant enzyme activities, osmoregulatory substances, and endogenous hormone contents in wheat during the seedling stage. Compared with no growth regulator spraying, spraying growth regulator under 150 mmol·L-1 saline-alkali stress increased the height of wheat seedlings by 7.69%-13.15%, 12.66%-15.50% and 1.01%-12.68%, respectively; root length by 7.44%-25.28%, 2.21%-19.62% and 30.38%-60.52%; stem fresh mass increased by 3.58% - 16.59%, 12.50% - 47.61% and 20.00% - 96.19%, respectively. Spraying growth regulators improved antioxidant enzyme activities of wheat seedlings compared with clear water treatment, among which spraying salicylic acid had the best effect in enhancing antioxidant enzyme activities of wheat seedlings. Under all saline-alkali stress, spraying salicylic acid and spermidine significantly increased the soluble sugar and soluble protein contents of wheat leaves by 13.19%-23.11%, 8.99%-27.65% and 3.18%- 28.76% and 7.50% - 29.31%, respectively, compared with that of clear water treatment. The growth hormone content of wheat leaves sprayed with salicylic acid was significantly higher than those of other treatments under 0, 100, and 150 mmol·L-1 saline-alkali stress; the gibberellin content was significantly increased by spraying salicylic acid under 50, 100 mmol·L-1 saline-alkali stress; the cytokinin content of the sprayed salicylic acid and spermidine treatments was significantly higher than that of 5-ALA under 50 and 150 mmol·L-1 salinealkali stress; the cytokinin content of the sprayed salicylic acid and spermidine treatments was significantly higher than that of 5-ALA under 0 and 100 mmol · L-1 saline-alkali stress, spraying 5-ALA significantly decreased the abscisic acid content and increased the cytokinin content of leaves. Salicylic acid and spermidine sprayed under saline-alkali stress promoted the seedling growth of wheat better than 5-ALA, which helped to alleviate the inhibitory effect of saline-alkali stress on its growth and improve the salinity tolerance of wheat. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
