|
| Isolation and identification of a strain of black soil straw degrading bacteria and optimization of culture conditions |
| Received:October 14, 2024 |
| View Full Text View/Add Comment Download reader |
| KeyWord:black soil;utilization of straw resources;cellulase;bacillus thuringiensis;Box Behnken response surface methodology |
| Author Name | Affiliation | E-mail | | ZHANG Lei | Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China
University of Chinese Academy of Sciences, Beijing 100049, China | | | LIU Tianyu | College of Horticulture, Jilin Agricultural University, Changchun 130118, China | | | YAN Chengzhao | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China | | | ZHANG Jinpeng | Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China | | | YU Yong | Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China | yuyong@iga.ac.cn |
|
| Hits: 180 |
| Download times: 178 |
| Abstract: |
| In order to achieve sustainable agricultural development and straw resource utilization, this study isolated, purified and identified a bacterial strain with strong straw degradation ability in the black soil area of northeast China, determined its cellulase enzyme activity and optimized its basic medium by Box-Behnken response surface method. After morphological, physiological, biochemical, and molecular biology identification, the strain was identified as Bacillus thuringiensis and named ZL-5. According to cellulase activity assay, the straw degradation ability of ZL-5 was significantly improved compared to the ZL-140, with a secretion of 6.76 U·mL-1. According to the Box Behnken response surface methodology experimental design, the maximum theoretical OD600 value per 20 mL of basic medium was 1.221 45 when the formula was sucrose 3.563 95 g·L-1, yeast extract 4.976 84 g·L-1, MgSO4 1.086 93 g·L-1, H2PO4 0.5 g·L-1, K2HPO4 1.5 g · L-1, NaCl 1.0 g · L-1. The screened strain ZL-5 has strong straw degradation ability, which can provide potential strain resources for biodegradation of straw. |
|
|
|
