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| Response characteristics of DNRA bacteria in paddy soil under wet and dry alternation |
| Received:September 05, 2024 |
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| KeyWord:paddy soil;alternating wet and dry;DNRA bacterial;nrfA;community structure |
| Author Name | Affiliation | E-mail | | LIU Jiaqi | College of Resources, Hunan Agricultural University, Changsha 410128, China | | | ZHANG Yi | College of Resources, Hunan Agricultural University, Changsha 410128, China | | | SUN Geng | Hunan Soil and Fertilizer Institute, Changsha 410125, China | | | NIE San'an | College of Resources, Hunan Agricultural University, Changsha 410128, China | sanie@hunau.edu.cn |
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| Abstract: |
| This paper aims at studying the response of DNRA bacteria community structure to dry and wet alternation in paddy soils with different fertilizers, and to analyze the response characteristics of soil DNRA bacteria to moisture management. Using paddy soil from longterm positioning test site, three treatments of single application of chemical fertilizer（NPK）, 30% organic fertilizer + 70% chemical fertilizer（OM）, and no fertilizer（CK）as control were selected with incubation experiments under alternating wet and dry（continuous wetting, continuous flooding, and alternating wet and dry）. Soil DNA was extracted after 23 d of incubation, and high-throughput sequencing of the DNRA functional gene nrfA was performed to investigate the response of the DNRA bacterial community structure to wet and dry alternation. The results showed that：the compositions and relative abundance of DNRA bacterial communities in paddy soils were changed to some extent. At the phylum level, wet-dry alternation decreased the relative abundance of Pseudomonadota, Verrucomicrobiota and Chloroflexota, and increased the relative abundance of Acidobacteriota. Continuous flooding favored the relative abundance of Chloroflexota but decreased the relative abundance of Actinomycetota. The relative abundance of Actinomycetota was higher under the continuous wetting treatment. Community α-diversity analysis showed that wet-dry alternation decreased Chao1 index, Shannon index and Simpson index of soil DNRA bacteria, but Shannon index and Simpson index were higher in continuously flooded soil than in wet treatment. Under the same water management conditions, there was no significant difference in Shannon index of DNRA bacteria in paddy soil between different fertilization treatments. The results of principal component analysis showed that the community structure of DNRA bacteria in paddy soil differed significantly among different moisture treatments. The community structure and α - diversity of DNRA bacteria in paddy soil responded to the moisture changes, but the response of α-diversity to different fertilizers was not significant. We hypothesize that the influence of moisture management on DNRA bacteria was greater than that of fertilizer management. |
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