|
| Effects of composite bacterial inocula on the reduction of antibiotic resistance genes during the membrane-covered composting of chicken manure |
| Received:May 31, 2024 |
| View Full Text View/Add Comment Download reader |
| KeyWord:chicken manure;membrane composting;ARGs;compound bacterial agent;stratified sample;nitrogen |
| Author Name | Affiliation | E-mail | | ZENG Jingli | College of Animal Science, South China Agricultural University, Guangzhou 510642, China | | | XIE Shiyin | College of Animal Science, South China Agricultural University, Guangzhou 510642, China | | | DENG Yiheng | College of Animal Science, South China Agricultural University, Guangzhou 510642, China | | | NIU Shihua | Wens Food Group Co., Ltd., Yunfu 527300, China | | | LI Youjian | Wens Food Group Co., Ltd., Yunfu 527300, China | | | XING Sicheng | Center for Microbial Communities Research in South China Agricultural University, Guangzhou 510642, China
Key Laboratory of Genomics and Molecular Breeding in Livestock and Poultry Animals in Guangdong Province, Guangzhou 510642, China
National Engineering Research Center for Breeding Swine and Poultry Industry Cooperative, Guangzhou 510642, China | | | LIAO Xindi | College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Key Laboratory of Genomics and Molecular Breeding in Livestock and Poultry Animals in Guangdong Province, Guangzhou 510642, China
National Engineering Research Center for Breeding Swine and Poultry Industry Cooperative, Guangzhou 510642, China
Guangdong Provincial Key Laboratory of Pig and Poultry Farming, Guangzhou 510642, China | xdliao@scau.edu.cn |
|
| Hits: 1993 |
| Download times: 1572 |
| Abstract: |
| To explore the pattern of antibiotic resistance genes（ARGs）reduction during the composting process of laying hen manur, mushroom residue was used as auxiliary material to mix with laying hens manure for conducting a composting test. The experimental design included a blank broth group（CK）, a commercial bacterial agent group（EM）, and two self-developed bacterial agent groups（PB4 andPB5）. Mixed samples were collected on days 0 and 30, and samples from the top, middle, and bottom layers were collected on day 30. The physical and chemical indexes and ARGs of the samples were analyzed, and the effects of three bacterial agents on the reduction of ARGs and nitrogen fixation during membrane composting were discussed. The results indicate that among the nine selected resistance genes and two mobile genetic elements in this study, in the mixed samples on day 30, compared with the initial absolute abundance of ARGs, the removal rates of bla-NDM and tetA in the EM group were significantly increased compared with the CK group（P<0.05）. Additionally, the removal rates of bla-NDM, ermC, intI2 and tetA in PB4 and PB5 groups were also significantly increased compared with the CK group（P< 0.05）. However, it was found that the removal effect of drfA1, intI1 and tetX in PB5 group was significantly lower than that in the CK group （P<0.05）. Furthermore, when comparing with their initial abundance levels, it was observed that using PB5 bacteria agent promoted an increase in tetX abundance. Similarly, using the EM bacteria agent led to an increase in the abundance of ermC, intI2 and tetX. The abundance of ermC, intI2 and tetA also increased in the CK group. Comparing the initial abundance of resistance genes within each group, the addition of PB4 bacteria agent removed 77.63% of the absolute abundance of total resistance genes, which was significantly higher than that of CK（67.31%）, EM（70.36%）and PB5（72.23%）. In stratified samples, EM bacteria agent exhibited the highest removal efficiency of antibiotic resistance genes（ARGs）in the bottom layer at 53.15%, while PB4 and PB5 bacteria agents achieved removal rates exceeding 90% in the upper and middle layers. However, PB5 bacteria agent increased the abundance of ARGs in the bottom layer. After composting, the addition of PB4 bacterial agent significantly increased the ammonium nitrogen content by 13.72%, while also reducing accumulated ammonia emission by 11.97%（P<0.05）compared to the CK group. However, the addition of EM bacteria agent and PB5 bacterial agent did not have a significant effect on reducing NH3 emission. Additionally, the content of nitrate nitrogen decreased with the addition of PB5 bacteria agent. In summary, the PB4 bacteria agent has the best comprehensive benefit for the reduction of ARGs and nitrogen fixation. |
|
|
|
