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| Determination of ochratoxin residual in crucian carp by UPLC-MS/MS |
| Received:December 04, 2024 |
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| KeyWord:Carassius auratus;ochratoxin;UPLC-MS/MS;pollution risk |
| Author Name | Affiliation | E-mail | | LI Chenxi | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | QIAN Zhisong | Hangzhou EXPEC Technology Co., Ltd., Hangzhou 311300, China | | | HE Mengai | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | LIU Zhiyu | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | XIE Xinyi | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | MI Yingqiu | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | PAN Ningning | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | HOU Jiayin | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | LI Meng | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | LIU Lianliang | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | | | CHEN Liezhong | State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China | zwsclz@163.com |
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| Abstract: |
| To explore detection techniques for ochratoxin B（OTB）and ochratoxin C（OTC）in fish meat, as well as precise detection techniques for ochratoxin A（OTA）, a new method was developed for simultaneous determination of OTA, OTB and OTC in crucian carp using ultra-high performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）, and the contamination of 3 toxins in 15 samples collected from Hangzhou market was determined. The results showed that the proposed method had a good linear relationship in the mass fraction range of 0.1~50 μg·kg-1, and average recovery rates of 1, 5 μg·kg-1 and 25 μg·kg-1 were 77.15%-107.59% through pretreatment and optimization of detection conditions. The OTA detection rate of 15 commercially available samples was 20%, and the highest detection value was 0.453 μg·kg-1. This method has good recovery rate and reproducibility, and is suitable for residual detection of ochratoxin in fish meat. |
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