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| Effects of Phanerochaete chrysosporium and Trichoderma longibrachiatum on pig manure-corn stalk composting and the structure of microbial community |
| Received:November 20, 2024 |
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| KeyWord:agricultural waste;aerobic composting;microbial agent;humification;bacterial community |
| Author Name | Affiliation | E-mail | | TIAN Jingjie | Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China | | | CUI Bingjian | Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China | | | LIU Chuncheng | Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China | | | SUN Jiuming | College of Agriculture, Henan University, Kaifeng 475001, China | | | CUI Erping | Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China | | | HU Chao | Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China | | | LIN Yanyu | Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China | | | LI Zhongyang | Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China | lizhongyang@caas.cn |
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| Abstract: |
| In order to achieve the efficient resource treatment of agricultural organic waste, the effects of inoculating Phanerochaete chrysosporium and Trichoderma longibrachiatum on nutrient changes, humification process and microbial community succession during composting were studied. In this experiment, pig manure and corn straw were used as composting raw materials, and four treatments were set up：no inoculation（CK）, inoculation of Phanerochaete chrysosporium（PC）, Trichoderma longibrachiatum（TL）and their mixed culture （CM）. By measuring indicators such as temperature, pH, seed germination index（GI）, total nitrogen（TN）, total phosphorus（TP）, total potassium（TK）and total nutrient content during the composting process, the effects of different exogenous microbial agents on the composting effect were compared. The high-throughput sequencing technology of 16S rRNA gene was used to analyze the differential changes in the bacterial community structure during the composting process with the addition of different microbial agents.It was found that the addition of different microbial agents could promote the temperature rise of the compost pile. The bacterial community structure did not change significantly at different stages of each composting treatment, but there were significant differences in relative abundance at the same stage. At the phylum level, the relative abundance of Actinobacteria in the treatment with the addition of the composite microbial agent was increased. At the genus level, the treatment with the composite microbial agent increased the relative abundance of SM1A02 in the later stage of composting. Pearson correlation analysis showed that among the first 33 genera, 27 genera were significantly correlated with temperature, pH, EC and E4/E6（P<0.05）. The results of RDA indicated that at the genus level, the changes in the bacterial community structure during the composting process with different treatments were related to different environmental factor parameters, and the main environmental factors affecting the bacterial community structure were temperature and pH（P<0.05）. In conclusion, the addition of microbial agents, especially the composite microbial agent, can increase the diversity of the microbial community, and can also promote the maturity of the compost to a certain extent and increase the total nutrient content of the compost pile. |
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