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| Impact of decomposing and nitrogen-fixing microbial inoculants on nitrogen transformation dynamics and diazotrophic community assembly during cotton stalk composting |
| Received:May 05, 2025 |
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| KeyWord:cotton straw;composting;decomposing and nitrogen-fixing microbial agents;nitrogen transformation;diazotrophic microbiota |
| Author Name | Affiliation | E-mail | | WANG Zhihui | Institute of Microbiology, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China | | | ZHOU Liuyan | Institute of Microbiology, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China | | | YANG Xinping | Institute of Microbiology, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China | | | LI Pengbing | Urumqi Comprehensive Experimental Station, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830031, China | | | YANG Bin | Institute of Microbiology, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China | | | XIE Yuqing | Institute of Microbiology, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China | xyqmail760530@163.com |
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| Abstract: |
| In order to explore the influence of inoculation of nitrogen-fixing bacteria on nitrogen transformation and nitrogen-fixing microbial community in cotton straw composting. In this study, cotton straw+Bacillus velezensis(TG1), cotton straw+Bacillus velezensis and Paenibacillus illinoisensis(TG2) and cotton straw(CK) were set up for composting, and the decomposition effect and the effect during composting were studied. The results showed that compared with CK, TG1 and TG2 treatments were longer and more stable at high temperature, which effectively increased the germination rate of seeds by 12.2% and 11.7%, respectively, and promoted the maturity and stability of compost. The contents of total kjeldahl nitrogen(TKN)in TG1 and TG2 treatments were 23.11 mg·kg-1 and 24.63 mg·kg-1, respectively, which were 17.2% and 24.9% higher than CK treatment. Further analysis showed that inoculation of rot-promoting nitrogen-fixing bacteria significantly affected the functional genes of nitrogen transformation and the succession of nitrogen-fixing bacteria community during composting. At the composting stage, the copy number of nifH gene in TG1 and TG2 treatments was 6.1% and 10.6% higher than that in CK treatment, while the copy number of denitrification function(nirS and nirK)gene was significantly lower than that in CK treatment(P<0.05). Inoculation treatment increased the abundance of Azotobacter(5 and 10 days), Pseudomonas(5, 10 and 40 days), Klebsiella(5 days), unclassified_f_Rhodospirillaceae(10 and 40 days)and unclassified_c_Alphaproteobacteria(3, 10 and 40 days), and obviously increased the relative abundance of unclassified_p_Proteobacteria. Molecular ecological network analysis showed that inoculation affected the network co-occurrence mode of nitrogen-fixing bacteria community during composting and changed the composition of key species. This study demonstrates that appropriate inoculation with functional microbial inoculants optimizes compost physicochemical properties, restructures diazotrophic communities, reduces nitrogen loss, enhances seed germination index, and ultimately enables environmentally sound valorization of agroforestry waste through improved composting efficiency and product quality. |
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