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| Effects of rice and shrimp on soil microbial community structure and functional characteristics in paddy field |
| Received:May 15, 2025 |
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| KeyWord:rice-crayfish co-culture;microorganism;metagenomic sequencing;community structure;functional gene |
| Author Name | Affiliation | E-mail | | DU Chunzhao | School of Geography and Environment, Jiangxi Normal University/Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Nanchang 330022, China | | | NI Caiying | School of Geography and Environment, Jiangxi Normal University/Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Nanchang 330022, China | ncy1919@126.com | | LIU Li | College of Urban Construction, Jiangxi Normal University, Nanchang 330022, China | | | JIANG Zhizhong | Yingtan Agricultural Technology Extension Center, Yingtan 335299, China | |
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| Abstract: |
| To elucidate the impact of the rice-crayfish co-culture system(DX)on soil microbial community structure and function, a metagenomic sequencing approach was employed to analyze the structural and functional diversity of the soil microbiota during the rice maturity stage, using traditional rice monoculture(CK)as the control. The results demonstrated that the DX system significantly enhanced soil microbial alpha diversity compared to CK(P<0.05). The dominant phyla in both systems were Pseudomonadota(25.34%-31.22%), Planctomycetota(10.39%-14.66%), Actinomycetota(10.84%-13.69%), and Chloroflexota(8.69%-11.48%), though their relative abundances varied. Notably, DX significantly increased the abundance of Pseudomonadota and Thermodesulfobacteriota(P<0.05). At the genus level, Singulisphaera(5.37%-7.99%), Bradyrhizobium(4.71%-5.35%), Ktedonobacter(2.30%-5.09%), and Pseudolabrys(2.73%-3.77%)were dominant in both soils. Compared with CK, DX significantly elevated the abundances of Bradyrhizobium, Pseudolabrys, Anaerolinea, and Methyloceanibacter. Correlation analysis indicated that soil pH and available potassium(AK)were significantly correlated with the relative abundance of major microbial taxa. KEGG(Kyoto Encyclopedia of Genes and Genomes)functional annotation revealed that metabolism was the predominant function of farmland soil microorganisms, accounting for 50.5%-51.23% of annotated functions. Compared to CK, DX increased the proportions of functional genes involved in carbon metabolism, two-component system, quorum sensing, and ABC transporters. Functional enrichment analysis further showed that the DX system enriched more pathways associated with metabolism, biosynthesis, and transport. COG(Clusters of Orthologous Groups)annotation confirmed that DX had significantly higher relative abundance in 8 out of the top 10 functional categories, 5 of which were related to cellular transport and metabolism. In conclusion, the rice-crayfish co-culture system significantly enhances soil microbial diversity, enriches functional gene repertoires, and strengthens the metabolic and transport potential of the soil microbial community. |
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