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| Impact of microplastic-antibiotic co-pollution on soil bacteriophages |
| Received:March 12, 2025 |
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| KeyWord:microplastics pollution;antibiotic pollution;bacteriophage;carbohydrate-active enzymes;antibiotic resistance genes |
| Author Name | Affiliation | E-mail | | LIU Shuo | College of Animal Science, South China Agricultural University, Guangzhou 510642, China | | | LI Haiyang | Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China | | | LI Yangkai | Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China | | | TANG Zixuan | Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China | | | NIU Shihua | Wens Foodstuffs Group Co., Ltd., Yunfu 527300, China | | | LIAO Xindi | College of Animal Science, South China Agricultural University, Guangzhou 510642, China
Wens Foodstuffs Group Co., Ltd., Yunfu 527300, China
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry Agriculture, Guangzhou 510642, China
Guangdong Key Laboratory of Livestock and Poultry Breeding, Guangzhou 510642, China | | | XING Sicheng | Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry Agriculture, Guangzhou 510642, China
Guangdong Key Laboratory of Livestock and Poultry Breeding, Guangzhou 510642, China | scxing@scau.edu.cn |
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| Abstract: |
| To investigate the impact of combined microplastic and manure-derived antibiotic pollution on soil phages, this study conducted soil incubation experiments, integrated with metagenomic sequencing and virome analysis, to systematically explore the composition, functional characteristics of soil phage communities, and their influence on ARGs dissemination under combined pollution conditions. The results demonstrated that while combined antibiotic-MP pollution did not significantly alter the compositional structure of soil phage communities, it led to a notable downregulation of phage functional gene expression. Key genes encoding functional enzymes such as glycoside hydrolases exhibited a 17%-20% reduction in expression compared to the uncontaminated control group. Simultaneously, combined pollution significantly promoted the enrichment and horizontal transfer of various ARGs, with the enrichment levels of some ARGs reaching 215.33% of those in the control group. Notably, in the soil carbon cycle, combined pollution further exacerbated phage-mediated ARGs transmission risks. Linear regression analysis revealed that phage dysfunction induced by combined pollution directly affected soil physicochemical properties, manifesting as a significant decrease in soil organic carbon content. The study indicates that combined pollution from microplastics and manure-derived antibiotics significantly impairs soil phage functionality, and increases the transmission risk of antibiotic resistance genes. |
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