|
| Effects of cadmium-contaminated soil at different concentrations on photosynthetic physiology and subcellular cadmium distribution in Ageratum conyzoides L. |
| Received:May 19, 2025 |
| View Full Text View/Add Comment Download reader |
| KeyWord:Ageratum conyzoides L.;phytoremediation;cadmium;subcellular distribution;photosynthetic system |
| Author Name | Affiliation | E-mail | | QIN Guimei | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China | | | XU Renzhi | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China | | | LIU Zhibo | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China | | | CHEN Deqian | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China | | | SONG Bo | College of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China
Collaborative Innovation Center for Water Pollution Control and Water Security in Karst Area of Guilin University of Technology, Guilin 541004, China | songbo@glut.edu.cn |
|
| Hits: 207 |
| Download times: 33 |
| Abstract: |
| Based on pot experiments, this study investigated the subcellular distribution characteristics of cadmium（Cd） and the response mechanisms of the photosynthetic system in Ageratum conyzoides L. under gradient Cd treatments. Pot experiments with varying Cd concentrations were conducted. We measured Cd accumulation traits, subcellular Cd distribution in leaves, chlorophyll fluorescence parameters, and performed micro-X-ray fluorescence（μ-XRF） mapping to analyze photosynthetic physiology and Cd distribution patterns. At the full-bloom stage, the fresh weight, plant height, and aboveground biomass of Ageratum conyzoides L. significantly exceeded those of the control（CK） group（P<0.05）, and the soil Cd content was 5.1 mg·kg-1（T1）, and the biomasss of the underground and aboveground parts reached the maximum value（P<0.05）. The Cd content in the aboveground part of plants increased significantly with the increase of soil Cd content and reached 421.19 mg·kg-1（P<0.05） when the soil Cd content was 60 mg·kg-1. Under different Cd content treatments, the aboveground transport coefficient（TF） of Ageratum conyzoides L. was >1, and the extraction amount of Cd was above 100 μg·plant-1, which was significantly higher than that of the CK（0.32 μg·plant-1, P<0.05）, showing superaccumulation characteristics. Subcellular Cd partitioning followed：the cell wall（61%-94%）>organelles > soluble fractions, identifying the cell wall as the primary Cd storage site, mitigating toxicity to mesophyll chloroplasts. μ-XRF revealed preferential Cd localization in leaf tips and veins, despite increasing leaf Cd accumulation, chlorophyll content remained stable. Elevated Fv/Fo（potential photochemical efficiency of PS Ⅱ） and Fv/Fm（maximum photochemical quantum yield of PS Ⅱ） indicated negligible Cd-induced damage to chlorophyll or PS Ⅱ function. Ageratum conyzoides L. shows high potential for in-situ remediation of moderately-to-highly Cd-contaminated soils. The study demonstrates that Ageratum conyzoides L. is applicable for in-situ remediation of moderately-to-highly Cd-contaminated soils and provides a theoretical foundation for enhancing subcellular detoxification mechanisms in hyperaccumulators. |
|
|
|
