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| Characteristics and microbial mechanisms of soil HONO emissions in protected vegetable greenhouses |
| Received:March 27, 2025 |
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| KeyWord:protected vegetable greenhouse;soil microorganisms;nitrogen cycle;HONO;functional genes |
| Author Name | Affiliation | E-mail | | ZHAO Yefei | College of Environmental Science and Engineering, Donghua University, Shanghai 201602, China | | | ZHANG Saiwei | Shanghai Academy of Environmental Sciences, Shanghai 200233, China | | | ZHOU Min | Shanghai Academy of Environmental Sciences, Shanghai 200233, China | | | LI Zhengwen | Shanghai Academy of Environmental Sciences, Shanghai 200233, China | | | WANG Hongli | Shanghai Academy of Environmental Sciences, Shanghai 200233, China | | | QIAN Xiaoyong | Shanghai Academy of Environmental Sciences, Shanghai 200233, China | qianxy@saes.sh.cn |
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| Abstract: |
| To investigate the relationship between soil microbial communities and gaseous nitrous acid（HONO）emissions in facility vegetable greenhouses, this study employed an online atmospheric HONO monitoring system to observe HONO emissions throughout the crop growth cycle. By analyzing the dynamic changes in soil physicochemical properties, soil microbial community structure, and soil microbial nitrogen-cycling functional gene abundances across four distinct stages of after sowing, after fertilization, before harvesting, and after harvesting, the soil microbial responses during the entire crop growth period was clarified, and the mechanism of enhancing HONO emissions by fertilization was explored. The results indicated that, during the experimental period, soil HONO emission fluxes ranged from -4.38 ng·m-2·s-1 to 35.25 ng·m-2·s-1. The mean flux before fertilization was（0.64±1.36）ng·m-2·s-1, while after fertilization it increased to （1.81±3.91）ng·m-2·s-1.Fertilization stimulated HONO emissions from greenhouse vegetable soils by accelerating soil acidification. The acidified environment promoted the conversion of NO-2 to HONO, thereby elevating soil-derived HONO release. Additionally, fertilization markedly increased the abundance of microbial functional genes, including AOA amoA, AOB amoA, and nirK/nirS. However, fertilization may inhibit microbial denitrification by elevating soil oxidation-reduction potential, thus making nitrification the dominant microbial process in the soil. This mechanism may promote NO-2 formation and accumulation through enhanced ammonia oxidation in microbial nitrification. The acidic conditions further facilitated NO-2 release into the atmosphere, ultimately increasing soil HONO emission fluxes. |
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