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| Optimization of the response surface and evaluation of application potential of Pleurotus eryngii residue fermented by Trichoderma citrinoviride GF-11 |
| Received:April 25, 2025 |
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| KeyWord:Trichoderma citrinoviride;Pleurotus eryngii residue;response surface methodology;application potential evaluation |
| Author Name | Affiliation | E-mail | | TANG Caiyan | College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education), Guiyang 550025, China
Guizhou Key Laboratory of Agricultural Microbiology, Guiyang 550025, China | | | YANG Changfa | College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education), Guiyang 550025, China | | | HU Qilan | College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education), Guiyang 550025, China | | | YUAN Yaya | College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education), Guiyang 550025, China | | | GE Yongyi | College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education), Guiyang 550025, China
Guizhou Key Laboratory of Agricultural Microbiology, Guiyang 550025, China | yongyige_09@163.com |
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| Abstract: |
| In an effort to procure microbial fertilizer through the fermentation of Pleurotus eryngii spent substrate by Trichoderma citrinoviride GF-11, a combination of the single-factor experimental approach and the response surface methodology was employed. The objective was to delineate the optimal sporulation conditions for the fermentation of Pleurotus eryngii spent substrate by this fungal strain. The activities of cellulase and laccase were determined by the 3, 5-dinitrosalicylic acid（DNS）method and the 2, 2′-azino-bis -（3- ethylbenzothiazoline-6-sulfonate）（ABTS）method respectively. The degradation effect was determined by the degradation rate method. The fermentation products were analyzed by LC-MS/MS to explore whether there were compounds with antibacterial and growth-promoting effects. The results revealed that four primary factors exerted a significant influence on the sporulation of Trichoderma citrinoviride GF-11 during the fermentation process, namely the concentration of sodium chloride, inoculum size, fermentation temperature, and fermentation duration. The optimal fermentation conditions were determined as follows：bran proportion of 50%, moisture content of 150%, sodium chloride concentration of 0.7%, inoculation amount of 12%, fermentation temperature of 28 ℃, and fermentation time of 10.6 days. Under these conditions, the spore yield reached 3.17×1010 cfu·g-1, representing a 4.52-fold increase compared to the initial conidia production when simply adding water and using an inoculation amount of 10% for fermenting Pleurotus eryngii residue. After fermentation, the exoglucanase activity, endoglucanase activity, and filter paper enzyme activity of the substrate were 0.506, 0.361 U·mL-1, and 0.764 U· mL-1, respectively. The laccase activity was 15.94 U·mL-1, and the degradation rate of the fermentation substrate reached 36.83%. Additionally, a diverse array of antibacterial and growth-promoting metabolites were generated, including auxin and benzoxazinone. This research indicates that the fermentation of Pleurotus eryngii residue by Trichoderma citrinoviride GF-11 yields a high number of spores. This microbial fertilizer has considerable application potential in both field and substrate fertilization. |
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