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| Isolation，characterization，and cadmium adsorption mechanism study of two cadmium-tolerant Pseudomonas aeruginosa strains |
| Received:February 06, 2025 |
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| KeyWord:Pseudomonas aeruginosa;cadmium pollution;characterization;adsorption mechanism |
| Author Name | Affiliation | E-mail | | WANG Dong | College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350000, China
National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350000, China | | | WAN Shangrou | College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350000, China
National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350000, China | | | XU Lixin | College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350000, China
National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350000, China | | | LIU Penghu | National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350000, China
College of Juncao and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350000, China | phliu1982@163.com |
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| Abstract: |
| To obtain efficient cadmium-adsorbing bacterial strains, two isolates（T1 and T2）were obtained from cadmium-contaminated soil and identified as Pseudomonas aeruginosa based on morphological characteristics and 16S rRNA sequence analysis. Growth curve analysis, adsorption experiments, SEM, FTIR, and XPS were used to compare their cadmium tolerance and adsorption mechanisms under different cadmium concentrations. Results showed that T2 exhibited earlier growth under low Cd2+ concentrations（0-30 mg·L-1）, while T1 showed better tolerance at higher concentrations（40-50 mg·L-1）. T2 demonstrated significantly higher cadmium adsorption rates（84.02%, 56.80%, and 32.83% at 50, 250, and 450 mg·L-1, respectively）and a greater total adsorption capacity（42.18 mg·g-1）compared to T1 （30.78 mg·g-1）. Mechanistic analysis revealed that T1 predominantly relied on intracellular accumulation（75.53%）, whereas T2 mainly employed extracellular adsorption（61.35%）. Functional group analysis indicated that hydroxyl, carboxyl, and ether groups played key roles in cadmium binding, with T2 possessing a higher density of surface-active sites. In summary, T2 showed superior cadmium adsorption performance, primarily through extracellular binding, offering valuable insights into the mechanisms of cadmium tolerance and adsorption in Pseudomonas aeruginosa. |
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