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Effects of copper and dicyandiamide on soil nitrification and N2O emission
Received:March 20, 2025  Revised:June 16, 2025
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KeyWord:copper;dicyandiamide nitrification inhibitor;NO3--N;N2O emission
Author NameAffiliationE-mail
WEN Yuhong College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China  
FENG Di College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China  
KONG Mengru College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China  
WAN Yunxing College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China  
ZHU Qilin College of Breeding and Multiplication, Hainan University, Sanya 572025, China  
WU Yanzheng College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China  
DAN Xiaoqian College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China  
TANG Shuirong College of Breeding and Multiplication, Hainan University, Sanya 572025, China  
AHMED S Elrys College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China  
ZHANG Jinbo College of Breeding and Multiplication, Hainan University, Sanya 572025, China  
MENG Lei College of Breeding and Multiplication, Hainan University, Sanya 572025, China menglei94@sohu.com 
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Abstract:
      To investigate the effect of dicyandiamide(DCD)nitrification inhibitor with copper(Cu)application on soil nitrification and N2O emission. In this study, two soils with different organic matter levels were set up for urea(U), urea with DCD(U_DCD), urea with DCD and addition of 10 mg · kg-1 Cu(U_DCD_Cu1)and 200 mg · kg-1 Cu(U_DCD_Cu2)treatments, respectively, in indoor incubation tests. The results showed that NO3--N content was significantly reduced by 30.79% and increased by 5.37%(P<0.05)in the U_DCD_Cu1 treatment compared to the U_DCD treatment in high and low organic matter soils, respectively. Compared with the U_DCD treatment, soil N2O emission was significantly increased by 25.48%(high organic matter)and 11.00%(low organic matter)in the U_DCD_Cu2 treatment. The synergistic effect of DCD and Cu was regulated by the soil organic matter content and Cu content:low content of Cu enhanced the inhibitory effect of DCD in high organic matter soils, and showed a weakening effect in low organic matter, and both Cu content both reduced the inhibition effect of DCD on N2O emission. This study reveals the mechanism of exogenously added Cu on DCD regulation of nitrogen loss in soils with different organic matter levels, and provides a theoretical basis for reducing soil N2O emissions.