|
| Mechanisms of doped nanoceria seed priming enhanced cold tolerance in rapeseed |
| Received:April 14, 2025 Revised:June 09, 2025 |
| View Full Text View/Add Comment Download reader |
| KeyWord:rapeseed(Brassica napus L.);nano-priming;cold stress;plant hormones;stress memory |
| Author Name | Affiliation | E-mail | | XU Shen | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | | | WU Yixin | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China | | | LI Hongzhuo | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China | | | GAO Yan | Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China | ygao@jaas.ac.cn |
|
| Hits: 573 |
| Download times: 382 |
| Abstract: |
| To investigate the potential of nano-priming technology in enhancing crop cold tolerance, we prepared two doped cerium oxide nanoparticles：manganese-doped cerium oxide nanoparticles（Mn-CeO2 NPs）and zinc oxide-doped cerium oxide nanoparticles（ZnOCeO2 NPs）. The regulatory effects and physiological mechanisms of the two materials on the germination of the cold-sensitive rapeseed variety Ningza 158 under cold stress were explored. Results showed that low temperature reduced germination potential and germination index by 18.5% and 46.2%, respectively. Mn-CeO2 NPs priming showed no significant effects on the germination characteristics of rapeseed seeds under low-temperature conditions. However, ZnO-CeO2 NPs priming significantly improved germination potential （increased by 26.9%）, germination index（increased by 11.1%）, and vigor index（from 760.2 to 788.1）. ZnO-CeO2 NPs enhance the cold tolerance of rapeseed seeds primarily through two pathways：ZnO-CeO2 NPs significantly increased the activity of β-amylase in seeds by 31.2%, promoting the degradation of stored starch and increasing soluble sugar content by 26.5%, providing osmotic protection and energy substrates for the seeds; Hormone metabolomics analysis showed that ZnO-CeO2 NPs significantly improved the levels of gibberellin 4 （increased by 133.6%）and salicylic acid（increased by 97.8%）in seeds under cold stress. These two hormones, through synergistic effects, respectively break physiological dormancy and initiate systemic resistance, enhancing the cold tolerance of rapeseed. |
|
|
|
