|
| Effects of long-term fertilization on denitrification-dependent anaerobic methane oxidation in paddy soils |
| Received:January 27, 2025 |
| View Full Text View/Add Comment Download reader |
| KeyWord:denitrifying methane anaerobic oxidation;reducing methane emissions in rice paddies;long-term fertilization;pig manure;straw residue return |
| Author Name | Affiliation | E-mail | | WANG Juan | College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, China
Agricultural Rural Carbon Peak Carbon Neutral Research Center, Chinese Academy of Agricultural Sciences(CAAS), Key Laboratory of Agricultural and Rural Ecological Environment, Ministry of Agriculture and Rural Affairs Institute of Agricultural Environment and Sustainable Development, CAAS, Beijing 100081, China | | | WU Juan | College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, China | | | LIAO Yulin | Soils and Fertilizer Institute of Hunan Province, Changsha 410125, China | | | LU Yanhong | Soils and Fertilizer Institute of Hunan Province, Changsha 410125, China | | | NIE Jun | Soils and Fertilizer Institute of Hunan Province, Changsha 410125, China | | | WAN Yunfan | Agricultural Rural Carbon Peak Carbon Neutral Research Center, Chinese Academy of Agricultural Sciences(CAAS), Key Laboratory of Agricultural and Rural Ecological Environment, Ministry of Agriculture and Rural Affairs Institute of Agricultural Environment and Sustainable Development, CAAS, Beijing 100081, China | | | QIN Xiaobo | Agricultural Rural Carbon Peak Carbon Neutral Research Center, Chinese Academy of Agricultural Sciences(CAAS), Key Laboratory of Agricultural and Rural Ecological Environment, Ministry of Agriculture and Rural Affairs Institute of Agricultural Environment and Sustainable Development, CAAS, Beijing 100081, China | qinxiaobo@caas.cn |
|
| Hits: 1172 |
| Download times: 537 |
| Abstract: |
| Denitrification-dependent anaerobic methane oxidation（DAMO）is a key process linking carbon and nitrogen cycles. A13C stable isotope labeling microcosm incubation experiment was conducted to investigate the effects of long-term fertilization on DAMO processes in red paddy soils. Four treatments were established：without fertilizer（CK）, chemical fertilizer（NPK：nitrogen, phosphorus, potassium）, combined chemical fertilizer and pig manure（NKM）, and chemical fertilizer with rice straw incorporation（NPKS）. Key findings include：total DAMO rates responded significantly to long-term fertilization. The maximum rate（1 762.93 nmol · g-1 · d-1）and minimum rate（25.01 nmol·g-1·d-1）were observed in the deep soil layer of NKM and the surface layer of CK, respectively. Notably, DAMO rates in the surface, middle, and deep soil layers of NKM were 276%, 447%, and 200% higher than those in corresponding NPKS layers（P< 0.05）. Nitrite-dependent DAMO（n-damo）rates increased with soil depth. The n-damo rate in the middle layer of NKM was 755% higher than that in NPKS（P<0.05）. Nitrate-dependent DAMO（Nr-damo）rates in the deep soil layer of NKM（702.49 nmol ·g-1·d-1）were significantly higher than those in the middle layer（38.77 nmol·g-1·d-1）（P<0.05）. Nr-damo rates increased with depth in CK but decreased in NPKS. 4）N2O-dependent DAMO（N2O-damo）rates were highest in surface soils（44.23-81.95 nmol·g-1·d-1）, followed by deep layers （8.47-18.46 nmol·g-1·d-1）, and lowest in middle layers（0.63-3.27 nmol·g-1·d-1）. Surface N2O-damo rates in NKM were significantly higher than those in NPKS（P<0.05）. This study demonstrates that pig manure combined with chemical fertilization（NKM）enhances DAMO activity more effectively than straw incorporation（NPKS）in red paddy soils. Additionally, deep soil layers exhibit higher n-damo potential, while surface soils show greater N2O-damo potential. |
|
|
|
