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| Microbial action mechanism of Tween 20 and biochar on methane produced by rice straw anaerobic fermentation system |
| Received:December 11, 2024 |
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| KeyWord:rice straw;anaerobic fermentation;additives;biomethane;microbial community |
| Author Name | Affiliation | E-mail | | LIU Jinrong | China Three Gorges University, Hubei International Scientific and Technological Cooperation Center of Ecological Conservation and Management in Three Gorges Area, Yichang 443002, China | | | LIU Yangyun | Hubei Zhengjiang Environmental Science and Technology Co., Ltd., Yichang 443002, China | | | YANG Feifan | China Three Gorges University, Hubei International Scientific and Technological Cooperation Center of Ecological Conservation and Management in Three Gorges Area, Yichang 443002, China | | | HUANG Yongwen | Hubei Zhengjiang Environmental Science and Technology Co., Ltd., Yichang 443002, China | | | CHEN Fangqing | China Three Gorges University, Hubei International Scientific and Technological Cooperation Center of Ecological Conservation and Management in Three Gorges Area, Yichang 443002, China | fqchen@ctgu.edu.cn |
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| Abstract: |
| To investigate the effects of additives on the microbial community in rice straw anaerobic fermentation systems, this study researched the effects of additives on biogas yield of the rice straw anaerobic fermentation system by adding Tween 20, biochar and their combination, respectively. The microbiological mechanism was revealed by measuring the biomethane yield as well as the microbial community diversity and structure of each treatment and analyzing the relationship between the biomethane yield and microbial community structure. The results showed that, the additives significantly increased the biomethane yield of the rice straw anaerobic fermentation system. The biomethane yield of TS（Tween 20+biochar）, T（Tween 20）and S（biochar）treatments increased by 390.67%, 182.07%, and 109.56% compared to the control, respectively. The additives significantly enhanced the diversity of bacterial and archaeal communities of the anaerobic fermentation system during the later stages. The Shannon diversity index of the bacteria community in TS, S, and T treatments increased by 28.16%, 26.45%, and 6.27% compared to CK, respectively, while the Chao1 richness index increased by 3.75%, 6.24%, and 24.31%. The Shannon index of the archaeal communities in S, TS, and T treatments was 11.58%, 5.62%, and 2.64% higher than CK, respectively. The additives also altered the composition and structure of dominant genera of bacterial and archaeal communities in the anaerobic fermentation system at the later stages, especially the archaeal community by increasing the relative abundance of Methanobrevibacter, Methanosphaera and Methanosarcina and decreasing the relative abundance of RumEn M2. In conclusion, three additive treatments enhance biomethane production in anaerobic fermentation systems by improving microbial community diversity during the late fermentation phase and optimizing the compositional structure of microbiota in rice straw digestion. This microbial remodeling reinforces methanogenic metabolic pathways and increases methane production efficiency, with the combined treatment of Tween-20 and biochar demonstrating the most significant improvement. |
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