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| Evolution characteristics of soil microbial communities in broccoli cultivation under biogas slurry application |
| Received:December 04, 2024 |
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| KeyWord:biogas slurry;broccoli;soil bacterial community;soil fungal community |
| Author Name | Affiliation | E-mail | | XU Jianfeng | Linhai Agricultural Technology Promotion Center, Linhai 317000, China | | | MENG Qingting | College of Animal Science and Technology · College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China | | | FU Jiajie | College of Animal Science and Technology · College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China | | | JIN Yuqing | Linhai Agricultural Technology Promotion Center, Linhai 317000, China | | | CAI Jianjun | Linhai Agricultural Technology Promotion Center, Linhai 317000, China | | | MA Jiawei | College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou 311300, China | jiawma@zafu.edu.cn | | WANG Mei | College of Animal Science and Technology · College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China | meiwang@zafu.edu.cn |
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| Abstract: |
| To investigate the impact of biogas slurry application on the diversity of bacterial and fungal communities in soils used for broccoli cultivation. Using metagenomic sequencing technology, we analyzed the changes in bacterial and fungal diversity under two treatments：combined application of chemical fertilizer and biogas slurry（MIX）, and sole application of biogas slurry（BS）. Combined with soil physicochemical properties, we further revealed the evolutionary patterns of microbial communities.The result shows that：Compared with the control（CK）, the BS treatment significantly increased soil pH, soil organic matter（SOM）, available potassium（AK）, and available phosphorus（AP）, with AK and AP content increasing by 633.0% and 95.3%, respectively. In contrast, the MIX treatment reduced soil pH but enhanced total nitrogen（TN）and AP levels. Microbial community analysis revealed that, in terms of bacteria, the BS treatment markedly increased the relative abundances of Pseudomonadota and Chloroflexota, with Chloroflexota showing a notable increase of 229.6% compared to CK. The MIX treatment, on the other hand, significantly enriched Bacteroidota. For fungi, the BS treatment significantly promoted the proliferation of Ascomycota, with a 148.7% increase in abundance, while the MIX treatment enriched Olpidiomycota populations in the soil. Correlation analysis indicated that the abundance of Sphingomonas was positively correlated with SOM and AK, while the abundance of Aspergillus increased with higher pH but was negatively correlated with alkaline hydrolysis nitrogen （AHN）and total phosphorus（TP）. In conclusion, the application of biogas slurry significantly improved soil pH, SOM, AK, and AP levels, while simultaneously optimizing the soil microbial community structure. These changes enhanced nutrient cycling and disease suppression potential in bacterial and fungal communities. As an environmentally friendly organic fertilizer, biogas slurry holds great promise for improving soil quality and promoting sustainable agricultural development. |
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