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| Screening，identification and degradation characterization of high-temperature-tolerant straw-degrading bacteria |
| Received:September 20, 2024 |
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| KeyWord:thermotolerant;straw degrading bacteria;identification;degradation characteristic;composite bacterial |
| Author Name | Affiliation | E-mail | | YIN Liwen | Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Provincial Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | MO Yingping | Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Provincial Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | GENG Tianzhi | Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Provincial Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | ZHANG Na | Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Provincial Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | LIU Cuiying | Jiangsu Key Laboratory of Agricultural Meteorology, School of Ecology and Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | FAN Jianling | Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Jiangsu Provincial Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China | jlfan@nuist.edu.cn |
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| Abstract: |
| The objective of this study was to identify high-temperature lignocellulosic-degrading bacteria from a paddy soil, establish a consortium of bacteria with high efficiency in lignocellulosic degradation, and investigate its degradation effect on straw, in order to promote the comprehensive utilization of agricultural waste. We obtained 11 degrading strains through preliminary screening by incubating for enrichment culture and using Congo red staining and aniline blue fading reaction. The enzymatic activities of cellulose, hemicellulose and lignin were measured. The highest β-D glucosidase activity was found in K6（716 U·mL-1）, the highest cellulase activity was obtained in K1（393 U· mL-1）, and the highest hemicellulase activity was found in K4（439 U·mL-1）. The highest lignin peroxidase activity was found in strain R3（6.213 U·mL-1）, the highest manganese peroxidase activity found in R1（3.989 U·mL-1）, while the highest laccase activity was found in strain K5（2.593 U·mL-1）. Based on the results of the transparent circle test, enzymatic activities of cellulose, hemicellulose and lignin, six strains（R2, R3, R4, K2, K5 and K6）were selected for molecular identification. The results showed that R2 was closely related to Priestia megaterium, R3 was closely related to Bacillus subtilis, R4 was closely related to Bacillus licheniformis, K2 was closely related to Priestia aryabhattai, K5 and K6 were closely related to Bacillus velezensis. Three consortia were constructed by using single strains. We found that consortium C（R4 and K6）had the best degrading effect, with the filter paper strip beginning to disintegrate on day 2 and turning into paper floss by day 5. After 15 days, the straw degradation rate was 23.69% higher than that of the control. The results suggest that the degradation effect of consortia was better than that of single bacterial colony. |
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