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| Metabolic response of Bacillus amyloliquefaciens A-3 under manganese ion stress |
| Received:June 21, 2024 |
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| KeyWord:manganese ion;Bacillus amyloliticus;untargeted metabolomics;metabolite |
| Author Name | Affiliation | | AO Jing | Microbial Research Institute of Liaoning Province, Chaoyang 122000, China
Key Laboratory of Microbial Strain Breeding of Liaoning Province, Chaoyang 122000, China | | LI Yang | Microbial Research Institute of Liaoning Province, Chaoyang 122000, China
Key Laboratory of Microbial Strain Breeding of Liaoning Province, Chaoyang 122000, China | | LIU Xiaohui | Microbial Research Institute of Liaoning Province, Chaoyang 122000, China
Key Laboratory of Microbial Strain Breeding of Liaoning Province, Chaoyang 122000, China | | SONG Liqun | Microbial Research Institute of Liaoning Province, Chaoyang 122000, China
Key Laboratory of Microbial Strain Breeding of Liaoning Province, Chaoyang 122000, China | | HU Qinqin | Microbial Research Institute of Liaoning Province, Chaoyang 122000, China |
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| Abstract: |
| In order to study the metabolic response of Bacillus amyloliquefaciens A-3 to Mn2+ stress, a sterile fermentation broth of A-3 was used as the research object. All metabolites of A-3 were identified by non-targeted metabolomics technology by adding Mn2+. According to the importance of variable weights（VIP>1）, the differentially expressed metabolites were screened by T-test（P<0.05）, and the A-3 metabolic pathway was enriched by KEGG database. The results showed that Mn2+ stress had a significant effect on the changes of metabolites in A-3 fermentation broth. A total of 332 differential metabolites were identified, 175 were detected in positive ion mode and 157 were detected in the negative ion mode. Among them, 114 metabolites were up-regulated, such as 3-methylindolepyruvate, spermine, adenosine and so on. Down regulation of 218, such as 5-hydroxylysine, citric acid, homogeneous acid and other small analytical organic acids. Through KEGG enrichment and topological analysis of metabolic pathways, 22 metabolic pathways with P<0.05 were found, among which the metabolic pathways of alanine, aspartate and glutamate were most significantly affected. The addition of Mn 2+ changes the metabolic level of A-3 cells, among which the expression of spermine is up-regulated. Spermine was involved in glutathione metabolism, pantothenate and CoA biosynthesis and other important metabolic and biosynthetic pathways. A-3 mainly responded to Mn2+ stress by regulating the expression of amino acids, nucleosides and small molecular organic acids. |
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