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| Construction of Cd phytoremediation microbial agent and its functional effects |
| Received:July 04, 2024 |
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| KeyWord:phytoextraction;oilseed rape;heavy metals;Cd-remediation agents;plant-microbe interaction;anti-oxidative system |
| Author Name | Affiliation | E-mail | | WANG Qiong | College of Environment and Ecology, Taiyuan University of Technology, Taiyuan 030024, China | | | FENG Xuewei | Shanxi Jinlan Judicial Appraisal Center for Environmental Damage, Taiyuan 030000, China | | | FENG Ying | College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China | yfeng@zju.edu.cn |
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| Abstract: |
| To elucidate the constructing principles of efficient Cd-remediation agents and its functional effects on oilseed rape, five PGPB strains isolated from the hyperaccumulating plant Sedum alfredii Hance were selected in this study, and the optimal mixed strain combinations were thereby determined by strain co-culture test, and combine it with the enrichment plant oilseed rape, to establish a plantmicrobial remediation system. After that, the effects of Cd-remediation agents inoculation with different concentration ratios on plant biomass, root development parameters, Cd uptake and accumulation, peroxide（H2O2） contents, glutathione（GSH） contents and antioxidative enzymatic activities were investigated. The results showed that the combination of the two PGPB strains, namely Bacillus sp. Bac1 and Burkholderia sp. Bac2 had the best co-culture growth condition and was more suitable as a Cd remediation agent. In addition, the functional effects of Cd-remediation agents on oilseed rape growth, Cd uptake, and accumulation varied due to the different inoculating concentration ratios of Cd-remediation agents. When the concentration ratio of Bac1 and Bac2 was 3∶1, inoculation of Cd-remediation agents was more favorable to the growth and development of oilseed rape and the Cd phytoextraction, while the antioxidant system of oilseed rape including antioxidative enzymatic activities, H2O2 contents, and GSH contents responded more strongly to Cd stress when the concentration ratio of Bac1 and Bac2 was 1∶1. |
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