|
| Toxic effect on lettuce induced by the coexistence of nanoplastics and arsenic in the soil and the related metabolic mechanisms |
| Received:March 12, 2024 |
| View Full Text View/Add Comment Download reader |
| KeyWord:soil;arsenic;nanoplastic;lettuce;toxic effect;metabolic mechanism |
| Author Name | Affiliation | E-mail | | FAN Weixin | School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China
Ministry of Agriculture and Rural Areas of Environmental Protection Research Monitoring, the Ministry of Agriculture and Rural Areas of Agricultural Products Quality and Safety Environmental Factor Control Key Laboratory, Tianjin 300191, China | | | QIU Chunsheng | School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China | qcs254@163.com | | MU Li | Ministry of Agriculture and Rural Areas of Environmental Protection Research Monitoring, the Ministry of Agriculture and Rural Areas of Agricultural Products Quality and Safety Environmental Factor Control Key Laboratory, Tianjin 300191, China | muli@caas.cn | | WENG Liangxian | Ministry of Agriculture and Rural Areas of Environmental Protection Research Monitoring, the Ministry of Agriculture and Rural Areas of Agricultural Products Quality and Safety Environmental Factor Control Key Laboratory, Tianjin 300191, China | | | GAO Ziwei | Ministry of Agriculture and Rural Areas of Environmental Protection Research Monitoring, the Ministry of Agriculture and Rural Areas of Agricultural Products Quality and Safety Environmental Factor Control Key Laboratory, Tianjin 300191, China | |
|
| Hits: 279 |
| Download times: 362 |
| Abstract: |
| The aim of this study was to explore the toxic effect on lettuce induced by the coexistence of nanoplastics and arsenic in the soil and the related metabolic mechanisms. Primarily, our investigation scrutinized the perturbations induced by nanoplastic introduction into arsenic-contaminated soil on soil pH, moisture content, and inorganic arsenic concentration alterations. Subsequently, we delved into elucidating the holistic impact of the synergistic interplay between nanoplastics and arsenic on lettuce growth dynamics, developmental processes, nutritional attributes, and antioxidative stress response. Finally, leveraging metabolomics analysis methodologies, we wished to delineate the mechanistic underpinnings of the toxicological effects of the nanoplastic-arsenic interaction on lettuce physiology. Our results proved that nanoplastic incorporation into arsenic-laden soil matrices increased soil pH concomitant with water-holding capacity and arsenic concentration reduction. At the culmination of the vegetative growth phase, the total arsenic content in soil devoid of nanoplastics declined by 6.85%, while that in 10 mg·kg-1 and 100 mg·kg-1 of nanoplastic-treated soils decreased by 9.87% and 20.33%, respectively. Notably, trivalent arsenic decreased more markedly. Furthermore, nanoplastics accentuated inorganic arsenic uptake by the lettuce plants, particularly during the nascent growth stages. In the 10 mg·kg-1 and 100 mg·kg-1 of nanoplastic-supplemented experimental groups, lettuce predominantly assimilated trivalent arsenic yielding a 30.25% and 39.10% increase, respectively. Notably, compared to the nanoplastic-untreated group, leaf fresh weight increased by 20.00% in the 10 mg·kg-1 group, albeit it declined by 4.00% in the 100 mg·kg-1 group on Day 15, suggesting a nuanced effect of low-concentration nanoplastics on plant vigor. However, at 30 days, both experimental cohorts displayed a decline of 6.15% and 10.77%, respectively, with analogous trends in lettuce root length, root weight, stem length, and chlorophyll content. At 45 days, they decreased by 6.33% and 12.66%, respectively. A marginal enhancement in cellulose, protein, and vitamin C content in lettuce was discerned in the nanoplastic-treated groups, accompanied by synchronous superoxide dismutase and malondialdehyde level increase, indicating an attenuated antioxidative stress response. Our metabolomic profiling demonstrated that nanoplastic inclusion in arsenic-contaminated soil matrices upregulated lactose, starch and sucrose, and inositol phosphate metabolism-related pathways. Concurrently, the tricarboxylic acid cycle as well as sulfur and nitrogen, tyrosine, and pyruvate metabolism-associated pathways were downregulated, deleteriously impacting plant physiological processes. |
|
|
|
