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| Effects of microcystin-LR on oxidative stress and intestinal microbiome in earthworms |
| Received:January 07, 2025 |
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| KeyWord:microcystins;Eisenia fetida;toxic effects;intestinal microbiome;soil contamination |
| Author Name | Affiliation | E-mail | | ZHAI Wenhua | Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China | | | MENG Jiaze | Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China | | | LI Yanwen | Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China | tlyw@jnu.edu.cn | | LIU Bailin | Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China | liubl2014@lzu.edu.cn | | MO Cehui | Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China | |
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| Abstract: |
| To investigate the toxic effects of Microcystin-LR（MC-LR）on soil fauna, this study employed a soil contact experiment, utilizing techniques such as enzyme activity assays, transmission electron microscopy, and high-throughput sequencing to examine the impacts of MC-LR contamination at varying concentrations（50, 150 μg·kg-1, and 500 μg·kg-1）on the antioxidant defense system and intestinal microbial community structure of earthworm（Eisenia fetida）. Results showed that MC-LR exposure damaged the muscular layers of the earthworms in a dose-dependent manner. In the high-concentration treatment, the content of MDA peaked（34.5 U·g-1）, while the activity of CL reached its lowest value（200.5 U ·g-1）. With the increase of MC-LR concentrations, both the T-AOC level and the GST activity in the earthworm increased firstly and then decreased. The α diversity of bacterial communities in the earthworm gut was significantly higher than that in the surrounding soil（P<0.05）, with Pseudomonadota, Actinobacteria and Bacillota being the dominant flora in the gut. The abundance of genetic information pathway and metabolic pathway of the earthworm gut microbial community were decreased by exposure to MC-LR. This study demonstrated that MC-LR exerts significant stress effects on the antioxidant system of earthworms, reduces their intestinal digestive capacity and gut microbial abundance, and consequently disrupts the normal transmission genetic information and the functioning of metabolic pathways in earthworms. |
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