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| Response of extracellular polymers in Proteucotlea chlorella to acetaminophen |
| Received:March 12, 2024 |
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| KeyWord:acetaminophen;Proteucotlea chlorella;extracellular polymers;chlorophylla |
| Author Name | Affiliation | E-mail | | ZHANG Huasheng | School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China
Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agro-Environment and Agro-product Safety, Tianjin 300384, China | | | LIU Yuemin | School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China
Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agro-Environment and Agro-product Safety, Tianjin 300384, China | lyuemn@126.com | | WANG Xiaorui | School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China | | | BAI Tao | School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China | | | ZHANG Xiaowen | School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China | |
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| Abstract: |
| To explore the different concentrations of acetaminophen（0-100 mg ·L-1, AAP）on chlorophyll a（Chl-a）and extracellular polymeric substances（EPS）of Proteucotlea chlorella, and the responses of Chl-a and EPS to AAP were analyzed by sampling on days 0, 2, 4, 6, 8, 10, and 12. The results showed that AAP could increase the content of chlorophyll a in Proteucotlea chlorella and promote the secretion of polysaccharides and proteins in EPS, which the change was concentration-dependent. Under the concentration of AAP with 100 mg·L-1, the content of polysaccharides and proteins in EPS increased from 223.83 and 47.02 μg·mL-1 to 361.81 and 70.12 μg·mL-1, respectively. Based on the analysis of EPS functional group changes by infrared spectroscopy, it was found that with the increase of AAP concentration, the intensity of the absorption peak representing C＝O increased significantly. At the same time, the absorption peaks at C＝O were shifted, and the absorption peaks of —OH groups had a similar trend, indicating that functional groups such as —OH and C＝O in EPS could bind to AAP. Three-dimensional fluorescence spectroscopy analysis showed that with the increase of AAP concentration, the fluorescence intensity of fulvic acids gradually decreased to disappear, and the fluorescence intensity of humic acids gradually increased, indicating that AAP could affect the composition of EPS chemical components of Proteucotlea chlorella. In conclusion, AAP can promote the synthesis of chlorophyll a and EPS in Proteucotlea chlorella algae, and the functional groups, such as fulvic acid, —OH and C＝O in EPS, may play an important role in the binding process with AAP. |
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