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| Effects of enhanced UV-B radiation on the diversity of endophytic microorganisms in tartary buckwheat leaves |
| Received:August 20, 2024 |
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| KeyWord:UV-B radiation;tartary buckwheat;high-throughput sequencing;endophyte;microbial diversity |
| Author Name | Affiliation | E-mail | | XIAO Huan | College of Agriculture and Biological Sciences, Dali University, Dali 671000, China | | | YANG Weiliang | College of Agriculture and Biological Sciences, Dali University, Dali 671000, China | | | KONG Qi | College of Agriculture and Biological Sciences, Dali University, Dali 671000, China | | | GONG Runlin | College of Agriculture and Biological Sciences, Dali University, Dali 671000, China | | | DUAN Yulian | College of Agriculture and Biological Sciences, Dali University, Dali 671000, China | | | ZHAO Yinzi | College of Agriculture and Biological Sciences, Dali University, Dali 671000, China | | | LUO Zonglong | College of Agriculture and Biological Sciences, Dali University, Dali 671000, China
College of Agriculture and Biological Sciences, Dali University, Yunnan Provincial Collaborative Innovation Center for Integrated Protection of Cangshan Mountain and Erhai Lake and Green Development of Basin, Dali 671000, China | | | FENG Yuan | College of Agriculture and Biological Sciences, Dali University, Dali 671000, China
College of Agriculture and Biological Sciences, Dali University, Key Laboratory of Agricultural Ecology of Erhai Lake Basin, Yunnan Provincial Department of Education, Dali 671000, China | fengyuancoral@dali.edu.cn |
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| Abstract: |
| This study aims to explore the mutualistic relationship between tartary buckwheat and endophytes in their ecological adaptation to UV-B radiation stress, thereby establishing a theoretical foundation for related research. Using high-throughput sequencing technology, we analyzed the effects of enhanced UV-B（280–320 nm）radiation on the diversity, community composition, and function of endophytes in tartary buckwheat leaves.The research was conducted using Yunqiao No. 2 as the subject. Natural light served as the control（CK）, while two UV-B radiation intensities（2.5 and 5.0 kJ·m-2）were applied over a period of 30 days. The diversity, community composition, and biological functions of endophytes in tartary buckwheat leaves were analyzed through high-throughput sequencing under enhanced UV-B radiation conditions. The results indicated that at a 97% sequence similarity level, 167 OTUs were identified among all sequenced samples of tartary buckwheat leaf endophytes, while 425 OTUs were identified for endophytic bacteria. The UV-B radiation treatment resulted in a reduction in the total number of OTUs for both tartary buckwheat leaf endophytes and endophytic bacteria, demonstrating the following trend：CK > 5.0 kJ·m-2 > 2.5 kJ·m-2. However, there was no significant effect on the diversity and abundance of endophytes（P > 0.05）. In all three treatment groups, the endophytic fungi were predominantly Ascomycota, with their relative abundance increasing alongside enhanced UV-B radiation. The dominant bacterial phyla included Proteobacteria and Fusobacteria, with Proteobacteria and Firmicutes being the common dominant phyla. The prevalent bacterial genera were unclassified_f_Alcaligenaceae and Pseudomonas, both of which showed a significant increase in relative abundance with enhanced UV-B radiation. One-way correlation network analysis revealed that increased UV-B radiation could promote synergistic symbiosis among endophytic bacterial communities in tartary buckwheat leaves, while weakening interspecific competition and interactions among endophytic fungal communities. Functional prediction and analysis of endophytic bacteria indicated that enhanced UV-B radiation led to a decrease in the relative abundance of fungal phytopathogenic species. Conversely, there was an increase in the abundance of endophytic bacterial genes associated with cell wall/cell membrane structure, lipid transport and metabolism, post-translational modification of proteins, and other related functions. Enhanced UV-B radiation resulted in significant changes in the composition and biological functions of endophytic bacterial communities in tartary buckwheat leaves, characterized by an increase in the number of dominant bacterial species and a heightened synergistic role among these communities. This may contribute to improving the ecological adaptability of tartary buckwheat to increased UV-B radiation. |
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