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| UPLC-MS/MS sample pretreatment methods for high-throughput determination of four groups of 81 antimicrobial residues in chicken manure |
| Received:February 28, 2024 |
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| KeyWord:chicken manure;ultra performance liquid chromatography-tandem mass spectrometry;antimicrobial drug;residue |
| Author Name | Affiliation | E-mail | | LIU Tian | College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030031, China
Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China | | | ZHAO Ruimin | College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030031, China
Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China | | | LI Muzi | Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China | | | LI Zehui | College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030031, China
Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China | | | SUN Rui | Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China
College of Veterinary Medicine, Beijing College of Agriculture, Beijing 102206, China | | | YANG Zhihe | Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China
College of Veterinary Medicine, Tianjin Agricultural College, Tianjin 300384, China | | | SUN Xiaoliang | Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China | | | ZHAO Sijun | Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China | | | SONG Cuiping | Chinese Center for Animal Health and Epidemiology, Qingdao 266032, China | | | HUO Nairui | College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030031, China | tgnrhuo@163.com |
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| Abstract: |
| An ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）method was developed for the highthroughput and rapid determination of 81 antimicrobial drug residues in chicken manure of four classes, including sulfonamides, macrolides, quinolones and tetracyclines, by comparing and optimising sample pretreatment methods.The samples were extracted with a mixture of EDTA-Mcllvaine and 10% acetonitrile/acetic acid（5∶15 V/V）, cleaned up and passed through the HLB solid phase extraction column, and then analyzed qualitatively and quantitatively in UPLC-MS/MS multiple reaction monitoring mode. The standard recovery test showed that the linear relationship of 81 drugs in chicken feces was good in the range of 1.0-100.0 μg·kg-1（R2>0.99）, the detection limit was 1.0-2.5 μg·kg-1, and the recovery rate was 60.24%-118.70%.The method is simple and reliable, has good extraction effect, and is suitable for screening and confirming the residues of many kinds of antibiotics in chicken manure. |
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