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| Influence of anaerobic fermentation-induced changes in dissolved organic matter from swine manure on the community structure of aquatic phytoplankton |
| Received:January 30, 2024 |
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| KeyWord:dissolved organic matter;cyanobacterial bloom;pyrosequencing;microbial community structure |
| Author Name | Affiliation | E-mail | | HUANG Jie | College of Environment and Ecology, Hunan Agricultural University, Changsha 410128, China | | | LIN Yiqing | College of Environment and Ecology, Hunan Agricultural University, Changsha 410128, China | | | SONG Xiaoming | Hunan Xiangjian Environmental Protection Technology, Changsha 410000, China | | | DAI Shiqin | College of Environment and Ecology, Hunan Agricultural University, Changsha 410128, China | | | WU Genyi | College of Environment and Ecology, Hunan Agricultural University, Changsha 410128, China | wugenyi99@163.com |
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| Abstract: |
| The purpose of this study is to investigate the impact of dissolved organic matter （DOM）from anaerobic fermentation treatment of pig manure on the growth of water phytoplankton and community structure. In this study, DOM was quantified using total organic carbon content, and its composition was analyzed through three-dimensional fluorescence spectra. High-throughput sequencing of 16S and 18S rRNA was employed to explore the community structure of prokaryotic and eukaryotic organisms in the water environment. The study compared the effects of pig manure-derived DOM before anaerobic fermentation（T1 treatment）and after anaerobic fermentation（T2 treatment）on phytoplankton growth and community structure. The results showed that chlorophyll-a content in the T1 treatment decreased from 3.69 mg·L-1 to 2.41 mg·L-1 then increased to 3.24 mg·L-1, but it continuously declined from 3.75 mg·L-1 to 1.59 mg·L-1 in the T2 treatment. DOM was mainly composed of five components, including microbial derivatives, typical terrestrial humic substances, tryptophan-like substances or dissolved polycyclic aromatic hydrocarbons, protein components, and tyrosine-like components. After anaerobic fermentation, the proportion of polycyclic aromatic hydrocarbon components in DOM increased significantly, affecting the growth of algae in the Chlorophyceae family. Additionally, the bioavailability of protein and tyrosine-like components increased, showing a negative correlation with the relative abundance of cyanobacteria. These results indicate that pig manure′ s DOM affects planktonic plant communities differently before and after anaerobic fermentation. And after anaerobic fermentation, it can reduce the biomass of planktonic plants. |
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