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| Effects of endophytes from Sedum alfredii on zinc uptake and transport in wheat |
| Received:January 12, 2024 |
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| KeyWord:hyperaccumulator;symbiotic microbes;microbial inoculum;Zn;biofortification |
| Author Name | Affiliation | E-mail | | LI Zhesi | Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China | | | LIAO Jiayuan | Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China | | | LIU Chanjuan | Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China | | | HUANG Lukuan | Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China | | | FENG Ying | Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, China | yfeng@zju.edu.cn |
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| Abstract: |
| In order to investigate the regulation mechanism of specific endophytes from hyperaccumulator plants on non-host crops zinc （Zn）nutrition, a pot experiment was carried out by using paddy soil and fluvo-aquic soil（FAS）, and 10 different endophytic bacteria treatments were set up to investigate the effect of endophytes on Zn uptake and transport in wheat. The results showed that in Zn-sufficient paddy soil, inoculation with SaPS1 increased shoot biomass by 158.48%, and inoculation with SaPA1 increased shoot Zn concentration by 108.83%. In Zn-deficient fluvo-aquic soil, inoculation with SaVA1 increased shoot biomass by 60.16%, and inoculation with SaZR8 and SaBR2 increased root Zn concentration by 74.17% and 73.02%. Real-time PCR showed that in paddy soil, the expression of TaZIP3, TaZIP6, TaZIP7, and TaZIP10 in shoots increased by more than 100% after inoculation with SaPA1 and SaPS1. In FAS, the expression of these four genes in both shoots and roots significantly increased after inoculation with SaBR2. Rhizosphere microbial communities sequencing of 16S rDNA revealed that although inoculation with SaPA1 and SaPS1 did not significantly affect the diversity of rhizosphere microbial communities, it altered the abundance of some functional microorganisms. These results suggest that specific endophytes from Sedum alfredii regulate Zn uptake and transport in wheat under different soil Zn conditions and are potential candidates for new Zn biofortification fertilizers. |
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