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| Effects of Chinese herbal residues on antibiotic resistance genes in vermicomposting with cow dung |
| Received:December 22, 2023 |
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| KeyWord:Chinese medicine residue;cow dung;vermicomposting;antibiotics resistance gene;transmission risk |
| Author Name | Affiliation | E-mail | | ZHAO Ran | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | HAN Bingjun | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | WANG Kuan | Tianjin Fuying Agricultural Science and Technology Co. Ltd., Tianjin 301701, China | | | ZHOU Xingzhi | Hong'an Agriculture and Rural Bureau, Hubei Province, Hong'an 438499, China | | | LI Xueping | Tianjin Fuying Agricultural Science and Technology Co. Ltd., Tianjin 301701, China | | | LI Xue | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIU Yiming | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | YANG Fengxia | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | yangfengxiacomeon@163.com | | DING Yongzhen | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | dingyongzhen@caas.cn |
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| Abstract: |
| Vermicomposting can reduce certain antibiotic resistance genes（ARGs）in animal manure, although residual ARGs are still present in worm casts. In this study, we performed vermicomposting with cow dung to investigate how adding 8 different Chinese herbal residues（3%）with varying antibacterial effects would affect the dynamic changes of antibiotic resistance genes（ARGs）and mobile genetic elements（MGEs）over 28 days. Our results indicated that different Chinese herb residues exerted varying effects on earthworm survival, with Radix Angelica Sinensis（DG）and Radix isatidis（BG）being favorable for their growth, while Wolfberry（GQ）, Sophora flavescens （KS）, Dandelion（PY）and Panax notoginseng（SQ）being detrimental to the same. ARGs were common in the compost substrate, with the dominant genes being sul-ARGs, tetX, and str-ARGs. Different Chinese herbal residues exerted distinct effects on the ARGs, with the abundance ranking on the 28th day as follows：DGsul-ARGs and str-ARGs with removal rates of 81.50%, 82.72%, and 72.02% for sul1 sul2, and strB, respectively. DG, ZS, and BG displayed high reduction rates（i.e., 84.76%, 79.72%, and 58.58%, respectively）for intI1 in the MGEs and a reduction rate of 100% for intI2, while other Chinese herbal residues promoted intI1 proliferation. In conclusion, our study indicated that different Chinese herb residues exert varying effects on ARGs and MGEs during vermicomposting, and selecting appropriate herb residues, such as angelica sinensis, could effectively reduce the risk of pollution. |
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