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| Effects of the rice-eel breeding model on the diversity and structure of soil ammonia-oxidizing microbial communities |
| Received:August 14, 2023 |
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| KeyWord:rice-eel breeding model;ammonia-oxidizing microorganism;high-throughput sequencing;soil physical and chemical property;microbial community |
| Author Name | Affiliation | E-mail | | CHU Xiangqian | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China | | | LÜ Weiguang | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China
Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China | | | FAN Haidan | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China | | | BAI Naling | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China
Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China | | | ZHENG Xianqing | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China
Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China | | | LI Shuangxi | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China
Key Laboratory of Integrated Rice-Fish Farming Ecosystem, Ministry of Agricuture and Rural Affairs, Shanghai 201403, China
Agricultural Environment and Farmland Conservation Experiment Station, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China | | | ZHANG Juanqin | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China
Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China | | | ZHANG Haiyun | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China
Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China | | | ZHANG Yue | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China
Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China | | | ZHANG Hanlin | Eco-environmental Protection Institute of SAAS, Shanghai 201403, China
Key Laboratory of Integrated Rice-Fish Farming Ecosystem, Ministry of Agricuture and Rural Affairs, Shanghai 201403, China
Agricultural Environment and Farmland Conservation Experiment Station, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China | zhanghanlinchick@163.com |
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| Abstract: |
| Research on the composition, structure, and diversity changes of soil ammonia-oxidizing microbial communities under the rice-eel breeding model is needed to gain in-depth insights into the impact on key microbes involved in soil nitrogen cycling. In this study, Illumina MiSeq high-throughput sequencing was systematically employed to analyze the community structure and diversity of ammoniaoxidizing microbes [ammonia oxidizing archaea（AOA）, ammonia oxidizing bacteria（AOB）and complete ammonia oxidizer（Comammox）] in the rice-eel breeding model. Two treatments were established：conventional rice field and rice-eel breeding treatments. Samples were collected from the central area and the edge of the ditch in each treatment, namely：conventional fertilization in the central area of rice fields （CCS）, conventional fertilization in the ditch edge area of rice fields（CMS）, rice-eel breeding model in the central area（ICS）, and riceeel breeding model in the ditch edge area（IMS）. The results showed that：compared to conventional rice field treatment, the rice-eel breeding model significantly reduced soil pH and decreased the difference in soil organic matter（SOM）and soil total N（TN）contents between the central and edge areas of the rice field, and increased soil potential nitrification rate（PNR）; In comparison to the conventional rice field treatment, the rice-eel breeding model markedly increased the abundance of AOA and Comammox Clade-A and Comammox Clade-B, while significantly decreasing the abundance of AOB. Meanwhile, IMS significantly enhanced the community diversity index of AOA, but reduced the community diversity index of AOB. AOA and Comammox promoted soil nitrification rates, while AOB did the opposite; Soil pH, SOM, and available nitrogen（AN）were identified as key environmental factors influencing ammonia-oxidizing microorganisms（P<0.05）; The rice-eel breeding model altered the structure of the ammonia-oxidizing microbial community and enhanced communication among these microorganisms, resulting in a more tightly connected internal network. The rice-eel breeding model, by altering soil environmental factors, especially soil pH, SOM, and AN content, significantly influences the composition, structure, and diversity of soil ammonia-oxidizing microbial communities. It enhances interactions among ammonia-oxidizing microbes and exerts a significant impact on key microbes involved in soil nitrogen cycling. |
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