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| Impact of microbial agents on the structure and function of the soil microbial community |
| Received:October 18, 2022 Revised:November 15, 2022 |
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| KeyWord:microbial agent;structure of soil microbial community;carbohydrate-active enzyme;antibiotic resistant gene;micro-ecological regulation |
| Author Name | Affiliation | | SHA Yuexia | Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750011, China | | HUANG Zeyang | Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750011, China | | LI Yunxiang | Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750011, China | | ZHAO Pei | Institute of Plant Protection, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750011, China |
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| Abstract: |
| This study explored the impact of microbial agents on the structure of the microbial community and functional genes in maize farmland. The metagenomic library was used to analyze the soil microbial community structure, gene abundance of nitrifying functional microorganisms, functional annotation and diversity of carbohydrate-active enzymes（CAZy）and antibiotic resistant genes（ARGs）. The results indicated that the abundance of soil microorganisms Proteobacteria, Actinobacteria, and Firmicutes increased after soaking the seed in Wugufengsu and combining it with microbial agents. In addition, the abundance of Acidobacteriotia, Gemmatimonadetes, Chloroflexi, and Candidatus Rokubacteria also decreased. The abundance of Thaumarchaeota and Euryarchaeota in archaea increased by 26.41% and 9.09%, and the abundance of Ascomycota, Basidiomycota and Chytridiomycota in fungi decreased. As for soil microbial genus, the relative abundance of Gemmatimona decreased by 9.76%, the abundance of Bacillus increased by more than 0.77 times, and the abundances of Nitrososphaera, Nitrospira and Candidatus_Nitrosocosmicus increased by more than 20%. Following the application of a microbial agent, the abundance of polysaccharide lyases（PLs）and carbohydrate binding modules（CBMs）increased. Furthermore, the abundance of bacitracin in ARGs genes decreased while the abundance of fluoroquinolone, chloramphenicol, aminoglycoside, beta-lactam increased. In particular, the gene abundance of ammonia oxidizing microorganisms（AOA and AOB）and nitrite oxidizing microorganisms（NOB）increased. The first two main components of PCA explained 98.78% of the class level CAZy difference and 59.33% of the sample level ARGs difference. NMDS and ANOSIM analysis results showed that there were differences in CAZy and ARGs resistance genes across treatments. In conclusion, soaking the seeds in Wugufengsu and combining them with microbial agents has the potential to significantly change the structure of soil microbial community in maize, increase the gene abundance of nitrifying functional microorganisms, optimize the proportion of soil microbial in CAZy, reduce bacitracin pollution in ARGs, and improve the micro-ecological environment of maize plant growth. |
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