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| Evaluation of garden biomass-degrading bacteria based on lignin monomer content |
| Received:December 23, 2020 |
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| KeyWord:garden biomass;lignin monomer;degradation strain;enzyme activity;degradation evaluation |
| Author Name | Affiliation | E-mail | | WU Tong | Key Laboratory of Soil Resource & Biotech Applications, Shaanxi Academy of Sciences, Xi'an Botanical Garden of Shaanxi Province (Institute of Botany of Shaanxi Province), Xi'an 710061, China
Xi'an University of Science and Technology, College of Geology and Environment, Xi'an 710043, China | | | LU Qiangqiang | Key Laboratory of Soil Resource & Biotech Applications, Shaanxi Academy of Sciences, Xi'an Botanical Garden of Shaanxi Province (Institute of Botany of Shaanxi Province), Xi'an 710061, China | luqiang@xab.ac.cn | | ZHAO Yezi | Key Laboratory of Soil Resource & Biotech Applications, Shaanxi Academy of Sciences, Xi'an Botanical Garden of Shaanxi Province (Institute of Botany of Shaanxi Province), Xi'an 710061, China
Xi'an University of Science and Technology, College of Geology and Environment, Xi'an 710043, China | | | CHEN Zhikun | Key Laboratory of Soil Resource & Biotech Applications, Shaanxi Academy of Sciences, Xi'an Botanical Garden of Shaanxi Province (Institute of Botany of Shaanxi Province), Xi'an 710061, China | | | XING Guoqiang | Xi'an Yanliang District Guoqiang Melon and Vegetable Cooperative, Xi'an 710089, China | | | JIA Ruiyu | Xi'an University of Science and Technology, College of Geology and Environment, Xi'an 710043, China | |
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| Abstract: |
| Based on the change in the lignin monomer content in the degradation residue, the enzymatic hydrolysis effect of garden biomass-degrading bacteria was evaluated. Using sodium carboxymethyl cellulose as the sole carbon source, functional screening and molecular identification of natural understory humus layer-degrading bacteria were conducted. The enzyme activity was optimized through orthogonal experiments and a degradation test was used to analyze the degradation weight loss rate. Gas chromatography-mass spectrometry was used to determine the lignin monomer content in the degradation residue and comprehensively evaluate the degradation efficiency. Strains QL-1 and QL-4, which had a higher enzyme activity, were Pseudomonas mandelii and Aspergillus fumigatus, respectively, and their cellulase activities were 14.10 U·mL-1 and 8.15 U·mL-1, respectively. The degradation rates of the two strains reached the highest levels of 9.48% and 13.91%, respectively, when they were cultured for 10 days, which were 2.49 times and 3.66 times greater than that of the blank control group, respectively. There was a significant reduction in the lignin monomer contents of the guaiacyl and syringyl units. The net degradation rate of the QL-4 test group was 15.57% at 10 days, which was 3.15 times greater than that of QL-1. The screened Aspergillus fumigatus had a stronger degradation activity, and the lignin monomer content in the degradation residues decreased to different degrees. |
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