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| Detection of lead （Ⅱ）in water using a dual-fluorophore molecular beacon |
| Received:May 24, 2020 |
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| KeyWord:lead ion (Ⅱ);dual-fluorophore;molecular beacon;aptamers;quantitative detection |
| Author Name | Affiliation | E-mail | | XIONG Wei-wei | Hubei Key Laboratory of Biologic Resources Protection and Utilization, School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, China | | | ZHANG Ying-kun | Enshi Polytecnic, Enshi 445000, China | | | LU Zi-jing | Hubei Key Laboratory of Biologic Resources Protection and Utilization, School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, China | | | WANG Peng | Hubei Key Laboratory of Biologic Resources Protection and Utilization, School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, China | | | ZHAI Kun | Hubei Key Laboratory of Biologic Resources Protection and Utilization, School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, China | zk3100@sina.com | | XIANG Dong-shan | Hubei Key Laboratory of Biologic Resources Protection and Utilization, School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi 445000, China | zk3100@sohu.com |
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| Abstract: |
| To develop a rapid and accurate method of measuring Pb2+ in water sources, a Pb2+-specific dual-fluorophore molecular beacon (MB)was constructed using two fluorophores(FAM and TAMRA)and the nucleic acid aptamer of Pb2+. A highly sensitive dual-color fluorescence quantitative detection method for Pb2+ in water was developed based on changes in the total fluorescence intensity of FAM and TAMRA after the molecular beacon and Pb2+ were combined. The results showed that the total fluorescence intensity(ΔIT)of FAM and TAMRA exhibited a good linear relationship with Pb2+ concentrations(C)ranging from 8×10-10 to 4×10-8 mol·L-1 under optimized conditions. The fitted regression equation was ΔIT=3.052C+13.129(R2=0.998 7), and the detection limit of the method was 1.5×10-10 mol·L-1(3σ, n=9). The results of this study indicate that the constructed dual-fluorophore molecular beacon can specifically recognize Pb2+ in water sources with high precision and accuracy and can therefore be applied in quantitative detection of Pb2+ in water. |
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